丛枝菌根真菌增强柏木幼苗抗干旱性研究  

Arbuscular mycorrhizal fungi can enhance the drought resistance of the seedlings of Cupressus funebris

作　　者：刘学松[1] 邵慰忠[1] 邓创 江波[3] 王志高 Liu Xuesong;Shao Weizhong;Deng Chuang;Jiang Bo;Wang Zhigao(Jiande Forestry Bureau,Hangzhou 311600,China;College of Forestry and Biotechnology,Zhejiang A&F University,Hangzhou 311300,China;Zhejiang Academy of Forestry,Hangzhou 310013,China)

机构地区：[1]建德市林业局,浙江杭州311600 [2]浙江农林大学林业与生物技术学院,浙江杭州311300 [3]浙江省林业科学研究院,浙江杭州310013

出　　处：《江苏林业科技》2024年第6期1-6,共6页Journal of Jiangsu Forestry Science & Technology

基　　金：浙江省与中国林业科学研究院省院合作林业科技项目“浙江省困难立地造林关键技术研究与示范”(2021SY08);浙江省“尖兵”“领雁”计划项目“受损森林生态系统快速修复和功能提升技术”(2022C02053)。

摘　　要：丛枝菌根真菌与植物形成共生体,有助于提高植物对环境的适应能力。通过对柏木进行摩西斗管囊霉(Fm)、幼套近明囊霉(Ce)和根内根孢囊霉(Ri)3种菌剂单独接种及其等量混合接种处理(MIX),测定和分析了连续干旱胁迫过程中柏木生长和生理指标变化。结果表明,混合接种组根系浸染率显著高于单菌种接种,分别是接种Fm、Ce和Ri的1.63,1.91,1.23倍;连续干旱胁迫下丛枝菌根真菌接种显著提高了柏木的株高和根系生长,混合接种处理组的株高增长量,根尖数和根表面积分别是CK的4.36,2.36,2.47倍;接种Fm,Ri,Ce的根表面积分别是CK的109.91%,131.90%,132.99%。丛枝菌根真菌处理提高了柏木的总叶绿素含量4.17%-9.13%、叶绿素a/b值平均提高6.74%;降低了干旱胁迫过程中细胞的伤害,MDA分别为CK的82.06%-94.06%,细胞保护性酶和渗透调节物质含量显著提升,POD含量分别为CK的116.58%-138.23%,SP含量分别为CK的104.56%-147.50%。结果显示,丛枝菌根真菌处理,尤其是混合接种处理,可以显著提高柏木对干旱胁迫的适应性,促进苗木生长,为干旱条件下的柏木造林提供了新的方法。Arbuscular mycorrhizal fungi(AMF)can establish symbiotic relationships with plants,significantly enhancing their capacity of adaptation to environmental conditions.Three AMFs,including Funneliformis mosseae(Fm),Claroideoglomus etunicatum(Ce),Rhizophagus intraradices(Ri)were separately inoculated on the root of Cupressus funebris and a mixed inoculation treatment(MIX)was also set up by mixing the three AMFs in equal quantities.The growth and physiological indicators of C.funebris under continuous drought stress were measured and compared.The results showed that the root colonization rate of the mixed inoculation group was significantly higher than that of the single inoculation groups,being 1.63,1.91 and 1.23 times that of the Fm,Ce,and Ri groups,respectively.Under the continuous drought stress,AMF inoculation could significantly improved the growth of C.funebris,with the growth by the mixed inoculation group being 4.36 times of CK in plant height,2.36 times in root tip number and 2.47 times in root surface area.The root surface area caused by Fm,Ri,and Ce was 9.91%,31.10%,and 32.99%larger than CK.The total chlorophyll content of C.funebris was increased by 4.17%to 9.13%by AMF treatment,and the average value of chlorophyll a/b was increased by 6.74%.The AMF treatment reduced cell damage during drought stress,with the MDA being 82.06%to 94.06%of that of CK.The content of protective enzymes and osmotic adjustment substances was significantly increased,with POD being 116.58%to 138.23%of that of and SP(soluble proteins)being 104.56%to 147.50%of that of CK.This study showed that that treatment with AMF,especially mixed inoculation,could significantly enhance the adaptability of C.funebris to drought stress and promote seedling growth.

关 键 词：丛枝菌根真菌 干旱胁迫 柏木 生长 生理 

分 类 号：Q948.122.2[生物学—植物学] Q949.32[农业科学—林木遗传育种] S723.9[农业科学—林学]