荧光寿命成像技术及其在生物医学领域的应用  

Fluorescence Lifetime Imaging Technology and Its Applications in Biomedical Science

作　　者：闫婉露 苏伟涛 陈大钦 YAN Wanlu;SU Weitao;CHEN Daqin(School of Science,Hangzhou Dianzi University,Hangzhou 310000,China;School of Physics and Energy,Fujian Normal University,Fuzhou 350000,China)

机构地区：[1]杭州电子科技大学理学院,浙江杭州310000 [2]福建师范大学物理与能源学院,福建福州350000

出　　处：《发光学报》2025年第1期46-59,共14页Chinese Journal of Luminescence

基　　金：浙江省自然科学基金项目(LZ22A040003)。

摘　　要：荧光寿命成像是一门在诸多领域都得到广泛应用的技术,该技术不仅具有高灵敏度、高时间分辨能力、空间分辨能力,且其测量不受激发光特性和细胞环境的影响,可定量化研究待测样品的荧光过程。由于上述优点,荧光寿命成像技术已经被广泛应用于生物医学领域。荧光寿命成像技术分为频域法和时域法,根据不同差频特征,频域法又可分为外差法和零差法,时域法主要有门控法、时间相关单光子计数法(TCSPC)和条纹相机测量法等三种实现途径。本文介绍了上述技术的基本原理,对荧光寿命成像技术在生物学研究、癌症肿瘤细胞的临床医学研究等领域的进展进行了综述,并对其未来的发展趋势进行了展望。Fluorescence lifetime imaging is a technique widely used in various fields.It has high sensitivity,high time resolution and spatial resolution.Moreover,it also can be used to quantitatively study the sample without the influence of excited laser and environmental factors.Because of the above advantages,fluorescence lifetime imaging had been widely used in biomedical field.Fluorescence lifetime imaging technique can be divided into frequency domain and time domain routines.Frequency domain routine can be divided into heterodyne method and homodyne method according to the difference frequency characteristics.There are three major methods to realize time-domain routine,i.e.gating method,time-correlated single photon counting method(TCSPC)and scanning camera measurement method.This work introduces the basic principles of aforementioned fluorescence lifetime imaging methods.We then review the recent application of fluorescence lifetime imaging in biological sciences and the clinic study of tumor cancer cell,and further give its perspectives.

关 键 词：荧光寿命成像技术 频域法 时域法 生物医学 

分 类 号：TN247[电子电信—物理电子学] O657.3[理学—分析化学]