基于香豆素的Zn^(2+)荧光探针构建及其应用  

Construction and Application of Zn^(2+)Fluorescent Probe Based on Coumarin

作　　者：王晔 朱鑫玥 怀玉 崔彤 李超 刘洋[1] 高云[1] 高妍[1] WANG Ye;ZHU Xinyue;HUAI Yu;CUI Tong;LI Chao;LIU Yang;GAO Yun;GAO Yan(College of Chemical Engineering,University of Science and Technology Liaoning,Anshan 114051,China)

机构地区：[1]辽宁科技大学化学工程学院,辽宁鞍山114051

出　　处：《发光学报》2025年第1期185-193,共9页Chinese Journal of Luminescence

基　　金：国家自然科学基金(U1910215);辽宁省科技厅项目(LNTP20231014607);国家级大学生创新创业训练计划项目(202310146036);辽宁省大学生创新创业训练计划项目(S202310146066);辽宁科技大学大学生创新创业训练计划项目(X202310146220)。

摘　　要：以香豆素醛和取代胺基的四氮唑为原料经缩合反应制备了新型荧光探针ASZ。通过高分辨率质谱(HR-MS)、核磁共振氢谱(1H NMR)和核磁共振碳谱(13C NMR)对其结构进行了表征。ASZ识别Zn^(2+)后最大吸收波长红移80 nm,可实现对Zn^(2+)的“裸眼”识别。且探针ASZ可通过荧光“OFF-ON”高选择性响应Zn^(2+),识别Zn^(2+)后探针的荧光强度增大4倍,其检测限低至9.94 nmol/L。利用HR-MS探讨了探针ASZ识别Zn^(2+)的响应机制。由于探针ASZ的水溶性较好,抗干扰性强,探针ASZ被用于水样中Zn^(2+)的半定量分析。最重要的是,ASZ因其生物毒性小且识别Zn^(2+)的灵敏度较高,已被成功用于细胞和小鼠体内Zn^(2+)的荧光成像。A novel fluorescent probe ASZ was prepared by condensation reaction of coumarin aldehyde and substituted amine tetrazolium.The structure was characterized by high resolution mass spectrometry(HR-MS),hydrogen nuclear magnetic resonance spectroscopy(1H NMR)and carbon nuclear magnetic resonance spectroscopy(13C NMR).The maximum absorption wavelength of ASZ was redshifted by 80 nm in the present of Zn^(2+),which can realize the“naked eye”recognition for Zn^(2+).The probe ASZ can identify Zn^(2+)with high selectivity by fluorescence“OFF-ON”respond,the fluorescence intensity of the probe increased by 4 times after the identification of Zn^(2+),the detection limit of Zn^(2+)by ASZ was 9.94 nmol/L.The response mechanism of probe ASZ for Zn^(2+)was investigated by using HR-MS.The probe ASZ was used for semi-quantitative analysis of Zn^(2+)in water samples because of its good water solubility and strong anti-interference.More importantly,ASZ was successfully utilized to confocal imaging of exogenous Zn^(2+)in HeLa cells and fluorescence imaging of exogenous Zn^(2+)in mice in vivo due to its advantages of low biological toxicity and high sensitivity for Zn^(2+).

关 键 词：荧光探针 香豆素 锌离子 生物成像 

分 类 号：O482.31[理学—固体物理]