Optical fiber biosensor with a bilayer bio-sensitive film for label-free detection of pseudomonas exotoxin A  

作　　者：Wanmei Guo Yongsen Yu Chao Xin Guangyong Jin 郭万美;于永森;辛潮;金光勇(Jilin Key Laboratory of Solid Laser Technology and Application,School of Science,Changchun University of Science and Technology,Changchun 130022,China;State Key Laboratory of Integrated Optoelectronics,College of Electronic Science and Engineering,Jilin University,Changchun 130012,China)

机构地区：[1]Jilin Key Laboratory of Solid Laser Technology and Application,School of Science,Changchun University of Science and Technology,Changchun 130022,China [2]State Key Laboratory of Integrated Optoelectronics,College of Electronic Science and Engineering,Jilin University,Changchun 130012,China

出　　处：《Chinese Optics Letters》2024年第11期36-41,共6页中国光学快报(英文版)

基　　金：supported by the National Natural Science Foundation of China (Nos.91860140,62090064,and U19A2077)。

摘　　要：A biosensor featuring an S-tapered fiber(STF) with a composite bio-sensitive film comprising graphene oxide and gold nanoparticles, has been proposed for the rapid, highly sensitive, and label-free detection of pseudomonas exotoxin(PE).The STF was created using a fusion splicer. Subsequently, the composite film and nanobody were successively assembled onto its surface. The detection mechanism relies on monitoring changes in the external refractive index induced by the specific binding of PE to the nanobody. The developed STF biosensor exhibited a remarkable sensitivity of 0.28 nm/(ng/mL)and a limit of detection as low as 0.21 ng/mL for PE.

关 键 词：optical fiber biosensor composite film nanobody:pseudomonas exotoxin. 

分 类 号：TB3[一般工业技术—材料科学与工程]