HPLC-MS/MS法检测大鼠体内ADB-BUTINACA及其代谢标记物动态分布及降解规律  

Determination of the Dynamic Distribution and Degradation of a Novel Synthetic Cannabinoid ADB-BUTINACA and Its Metabolic Markers in Rats by HPLC-MS/MS

作　　者：蔡玉刚 吴永富 周达江[2] 杨剑峰 杜颍川 马琦鸿 代勇[4] 王燕军[5] CAI Yugang;WU Yongfu;ZHOU Dajiang;YANG Jianfeng;DU Yingchuan;MA Qihong;DAI Yong;WANG Yanjun(Institute of Forensic Science,Luzhou Public Security Bureau,Luzhou 646000,Sichuan,China;Institute of Forensic Science,Chengdu Public Security Bureau,Chengdu 610017,China;Institute of Forensic Science,Yibin Public Security Bureau,Yibin 644000,Sichuan,China;Sichuan Police College,Luzhou 646000,Sichuan,China;Criminal Investigation Division of Sichuan Provincial Public Security Department,Chengdu 610015,China)

机构地区：[1]泸州市公安局刑事科学技术研究所,四川泸州646000 [2]成都市公安局刑事科学技术研究所,成都610017 [3]宜宾市公安局刑事科学技术研究所,四川宜宾644000 [4]四川警察学院,四川泸州646000 [5]四川省公安厅刑侦局,成都610015

出　　处：《刑事技术》2025年第1期74-80,共7页Forensic Science and Technology

基　　金：四川省省级大学生创新创业训练项目(S202112212073);四川省公安厅厅级科研项目(2021YYCX02)。

摘　　要：本文对烷基甲酰吲唑类合成大麻素ADB-BUTINACA在大鼠体内分布及降解规律进行探索。通过前期动物实验研究发现,ADB-BUTINACA在大鼠体内的代谢标记物为脱烷基代谢标记物:N-(1-氨甲酰基-2,2-二甲基丙基)-吲唑-3-甲酰胺(下称A1)和水解代谢标记物:3,3-二甲基-2-[1-丁基-1H-吲唑-3-甲酰胺基]丁酸(下称A2)。实验模拟吸毒人员口服大麻制品建立大鼠经灌胃ADB-BUTINACA染毒模型,用高效液相色谱-三重四极杆串联质谱仪(HPLC-MS/MS)检测各组织、血液、尿液和大便中ADB-BUTINACA及代谢标记物A1和A2的浓度,监测ADB-BUTINACA和代谢标记物在大鼠体内分布降解规律。结果发现ADB-BUTINACA进入大鼠体内后,迅速代谢并分布于各组织和血液,在12 h内呈不同规律分布及降解。在对1~10 d尿液和大便的监控中,ADB-BUTINACA及其代谢标记物仅在少量尿液中检出,在24~72 h的大便中均检出ADB-BUTINACA及其代谢标记物。目前,国内外对ADB-BUTINACA在大鼠体内的法医毒理学研究未见报道,本文建立的大鼠染毒模型,监测ADB-BUTINACA及代谢标记物在大鼠体内的分布及降解规律为体内ADBBUTINACA检验提供科学依据和数据参考。The distribution and degradation of the synthetic cannabinoid ADB-BUTINACA of alkyl formyl indazoles in rats were explored in this paper.Through previous animal studies,it was found that the metabolic markers of ADB-BUTINACA in rats were dealkylation metabolic markers:N-(1-aminoformyl-2,2-dimethylpropyl)-indazole-3-formamide(hereinafter referred to as A1)and hydrolytic metabolic marker:3,3-dimethyl-2-[1-Butyl-1H-indazole-3-formamido]butyric acid(hereinafter referred to as A2).An intoxication model of rat administered with ADB-BUTINACA by gavage established by simulating oral administration of cannabis products,and the concentration of ADB-BUTINACA and metabolic markers A1 and A2 in tissues,blood,urine and feces were detected by high performance liquid chromatography-triple quadrupole tandem mass spectrometry(HPLC-MS/MS),and the distribution and degradation of ADB-BUTINACA and metabolic markers in rats were monitored.The results showed that ADB-BUTINACA was rapidly metabolized and distributed in various tissues and blood after entering the rat body,and showed different regularity of distribution and degradation within 12 hours.In the monitoring of urine and feces collected within 1 to 10 days,ADB-BUTINACA and its metabolic markers were detected only in a small amount of urine,while they were all detected in feces collected within 24 to 72 hours.The rat intoxication model established in this study monitors the distribution and degradation of ADB-BUTINACA and metabolic markers in rats,providing scientific basis and data reference for the test of ADB-BUTINACA in vivo.

关 键 词：法医毒理学 ADB-BUTINACA 高效液相色谱-三重四极杆串联质谱 降解规律 动态分布 

分 类 号：DF795.1[医药卫生—法医学]