针刺预处理对缺血性脑卒中神经细胞铁死亡的影响  

Effect of acupuncture pretreatment on nerve cell ferroptosis in ischemic stroke

作　　者：高莹[1,2] 杨建[1,2] 石江伟[1,2] 薛静[1,2] 尚晓玉[1,2] 段洪涛 王海荣[1,2] GAO Ying;YANG Jian;SHI Jiangwei;XUE Jing;SHANG Xiaoyu;DUAN Hongtao;WANG Hairong(The First Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300183,China;National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion,Tianjin 300183,China)

机构地区：[1]天津中医药大学第一附属医院,天津市300183 [2]天津中医药大学国家中医针灸临床医学研究中心,天津市300183

出　　处：《中国动脉硬化杂志》2025年第1期24-29,共6页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金项目(82104998)。

摘　　要：[目的]研究针刺预处理对缺血性脑卒中大鼠神经细胞铁死亡的调控作用。[方法]将60只SD大鼠随机分为假大脑中动脉栓塞(MCAO)组、MCAO组和针刺+MCAO组。针刺+MCAO组选取人中穴、内关穴、三阴交穴进行针刺预处理,每天1次,共5天;预处理后行MCAO或假MCAO模型制备。Zausinger六分法对大鼠神经功能进行评分,TTC染色法计算脑组织梗死面积;电镜下观察脑组织病理形态学改变;比色法检测铁含量,ELISA法测定丙二醛(MDA)、谷胱甘肽(GSH)的含量,免疫荧光检测谷胱甘肽过氧化物酶4(GPX4)表达。[结果]与假MCAO组相比,MCAO组神经功能评分降低,梗死面积显著增加,电镜下线粒体数量减少,线粒体内嵴结构断裂、空泡化,脑组织铁蛋白、MDA含量升高,GSH含量和GPX4表达降低。与MCAO组相比,针刺+MCAO组神经功能评分升高,梗死面积降低,电镜下线粒体数量增多,结构清晰,部分线粒体内嵴结构减少或排列紊乱,脑组织铁蛋白、MDA含量降低,GSH含量和GPX4表达升高。[结论]针刺预处理可减轻大鼠神经功能损伤,其原因可能与调控铁蛋白、GSH、MDA含量和GPX4表达,提高细胞抗氧化能力,抑制神经细胞铁死亡有关。Aim To study the regulatory effect of acupuncture pretreatment on ferroptosis of nerve cells in rats with ischemic stroke.Methods Sixty SD rats were randomly divided into sham middle cerebral artery occlusion(MCAO)group,MCAO group,and acupuncture+MCAO group.In the acupuncture+MCAO group,the acupuncture points of DU26,PC6,and SP6,were selected for acupuncture pretreatment,once a day for a total of 5 days.After pretreatment,MCAO or sham MCAO models were prepared.The Zausinger sextintegral method was used to score the neurological function of rats,and the infarct volume of brain tissue was calculated by TTC staining.Electron microscopy was used to observe the pathomorphological changes of brain tissue.The iron content was detected by colorimetric method,the content of malondialdehyde(MDA)and glutathione(GSH)was determined by ELISA,and the expression of glutathione peroxidase 4(GPX4)was detected by immunofluorescence.Results Compared with the sham MCAO group,the MCAO group had a decrease in neurological function scores,a significant increase in infarct volume,a decrease in the number of mitochondria under electron microscopy,a rupture and vacuolization of the inner mitochondrial cristae,an increase in the contents of iron and MDA in brain tissue,and a decrease in GSH content and GPX4 expression.Compared with the MCAO group,the acupuncture+MCAO group had an increase in neurological function scores,a decrease in infarct volume,a large number of mitochondria under electron microscopy,a clear structure,a decrease or disordered arrangement of some mitochondrial crest structures,a decrease in the contents of iron and MDA in brain tissue,and an increase in GSH content and GPX4 expression.Conclusion Acupuncture pretreatment can alleviate neurological damage in rats,and its mechanism may be related to regulating iron,GSH and MDA contents in brain tissue,and GPX4 expression,improving cell antioxidant capacity and inhibiting nerve cell ferroptosis.

关 键 词：针刺 预处理 缺血性脑卒中 铁死亡 神经细胞 

分 类 号：R245.31[医药卫生—针灸推拿学] R741[医药卫生—中医临床基础]