氨基酸维生素对沙漠干热环境大鼠运动骨骼肌损伤保护作用研究  

Study on the protective effect of amino acid vitamins on skeletal muscle injury in rats in dry and hot desert environment

作　　者：武堂晓 尹紫依 吴荧秋 吴吉利 惠华强[1,2] WU Tangxiao;YIN Ziyi;WU Yingqiu;WU Jili;HUI Huaqiang(School of Public Health,Xinjiang Medical University,Urumqi 830011,China;General Hospital of XinjiangMilitary Command,Urumqi 830011,China;Shihezi University School of Medicine,Shihezi 832061,China)

机构地区：[1]新疆医科大学公共卫生学院,新疆乌鲁木齐830011 [2]新疆军区总医院,新疆乌鲁木齐830011 [3]石河子大学医学院预防医学系,新疆石河子832061

出　　处：《海南医科大学学报》2025年第1期25-32,共8页Journal of Hainan Medical University

基　　金：自治区区域协同创新专项(科技援疆计划)(2022E02121);新疆维吾尔自治区研究生科研创新项目资助(XJ2023G190)。

摘　　要：目的:观察大鼠在干热环境力竭运动及给予氨基酸维生素后,大鼠骨骼肌组织病理形态学和骨骼肌损伤相关等指标,探讨氨基酸维生素对干热环境大鼠运动骨骼肌损伤的保护作用。方法:将36只SPF雄性SD大鼠随机分为空白对照组、常温运动组、干热运动组、低剂量组、中剂量组、高剂量组,运动结束后取大鼠比目鱼肌组织观察病理学变化。ELISA检测血清炎症因子指标MCP-1;氧化应激指标CAT、GSH-Px;qRT-PCR检测大鼠比目鱼肌Nrf2/HO-1/NQO1 mRNA相对表达量;WB检测大鼠比目鱼肌Nrf2、HO-1、NQO1蛋白表达水平。结果:(1)大鼠核心体温:力竭运动后较运动前升高(P<0.05),低、中、高剂量组核心体温较干热运动组下降(P<0.05);(2)大鼠力竭时间:干热运动组力竭时间较常温运动降低(P<0.05),低、中、高剂量组力竭时间较干热运动组升高(P<0.05);(3)炎症因子及氧化应激:干热运动组血清MCP-1较常温运动组升高(P<0.05),MCP-1中、高剂量组较干热运动组降低(P<0.05);干热运动组血清CAT和GSHPx水平较常温运动组降低(P<0.05);CAT高剂量组较干热运动组升高(P<0.05),GSH-Px低、中、高剂量组较干热运动组升高(P<0.05);(4)qRT-PCR检测:干热运动组Nrf2、NQO1 mRNA相对表达水平显著较常温运动组降低(P<0.05),Nrf2、NQO1相对表达水平低、中、高剂量组较干热运动组升高(P<0.05),HO-1相对表达水平中、高剂量组较干热运动组升高(P<0.05);(5)WB检测:干热运动组Nrf2、HO-1蛋白表达量较常温运动组降低(P<0.05),Nrf2表达量低、中、高剂量组较干热运动组升高(P<0.05),HO-1表达量中、高剂量组较干热运动组升高(P<0.05),NQO1高剂量组较干热运动组升高(P<0.05)。结论:干热环境可能会加重运动骨骼肌损伤,氨基酸维生素可能对干热环境大鼠运动骨骼肌损伤的严重程度发挥保护作用。Objective:The histopathological morphology of skeletal muscle and the related indexes of skeletal muscle injury in rats were observed,and the protective effect of amino acid vitamins on sports skeletal muscle injury in rats in dry and hot environment was investigated.Methods:Thirty-six SPF male SD rats were randomly divided into blank control group,room temperature exercise group,dry heat exercise group,low dose group,medium dose group and high dose group.ELISA was used to detect serum inflammatory factor MCP-1.oxidative stress indicators CAT,GSH-Px;The relative expression levels of nuclear factor erythroid-2 related factor 2 and Nrf2HO-1NQO1 mRNA in rat soleus muscle were detected by qRT-PCR.WB was used to detect the protein expression levels of Nrf2,HO-1 and NQO1 in rat soleus.Results:(1)The core body temperature of rats increased after exhaustion exercise compared with that before exercise(P<0.05),and the core body temperature of the low,medium and high dose groups decreased compared with that of the dry heat exercise group(P<0.05);(2)Exhaustion time of rats:the exhaustion time of the dry heat exercise group was lower than that of normal temperature exercise(P<0.05),and the exhaustion time of low,medium and high dose groups decreased compared with that of normal temperature exercise(P<0.05).The exhaustion time of the high-dose group was higher than that of the dry-heat exercise group(P<0.05);(3)Inflammatory factors and oxidative stress:the serum MCP-1 of the dry-heat exercise group was higher than that of the room-temperature exercise group(P<0.05),the MCP-1 medium-dose and high-dose groups were lower than those of the dry-heat exercise group(P<0.05),and the serum levels of CAT and GSH-Px in the dry-heat exercise group were lower than those of the room-temperature exercise group(P<0.05).The high-dose CAT group was higher than that of the dry-heat exercise group(P<0.05),and the low,medium,and The mRNA expression levels of Nrf2 and NQO1 in the high-;dose exercise group were significantly lower than those in the

关 键 词：氨基酸维生素 干热环境 运动骨骼肌损伤 氧化应激 保护作用 

分 类 号：R873[医药卫生—运动医学]