柠条锦鸡儿WRKY28-2两个转录本对非生物胁迫的响应  

Abiotic Stress Response of Two Transcripts of WRKY28-2 from Caragana korshinskii

作　　者：李媛 李国婧[1] 王瑞刚[1] 万永青[1] LI Yuan;LI Guojing;WANG Ruigang;WAN Yongqing(Key Laboratory of Plants Adversity Adaptation and Genetic Improvement in Cold and Arid Regions of Inner Mongolia,Inner Mongolia Agricultural University,Hohhot 010018,China)

机构地区：[1]内蒙古农业大学旱寒区植物逆境适应与遗传修饰改良自治区重点实验室,呼和浩特010018

出　　处：《中国生物工程杂志》2024年第12期1-12,共12页China Biotechnology

基　　金：国家自然科学基金(31860217);内蒙古自治区重点研发和成果转化计划(科技合作)(2023KJHZ0013);内蒙古自治区高等学校碳达峰碳中和研究专项(STAQZX202310)资助项目。

摘　　要：可变剪接也叫选择性剪接(alternative splicing,AS),是指从一个mRNA前体中通过不同剪接方式产生不同mRNA剪接异构体的过程。WRKY转录因子是最大的转录因子家族之一,广泛参与生长、发育、衰老及非生物胁迫过程。研究发现柠条锦鸡儿(Caragana korshinskii Kom.)WRKY28-2基因会发生可变剪接,产生两个不同的转录本,即CkWRKY28-2和CkWRKY28-2-AS。CkWRKY28-2起始密码子为ATG,终止密码子为TGA,编码265个aa,蛋白质分子质量为30028.18 Da,pI为8.16;CkWRKY28-2-AS起始密码子为ATG,终止密码子为TGA,编码68个aa,蛋白质分子质量为7286.71 Da,pI为3.98。RT-qPCR分析结果表明,CkWRKY28-2主要在根和茎中表达,而CkWRKY28-2-AS主要在叶中表达;在干旱胁迫下CkWRKY28-2的表达量增加而CkWRKY28-2-AS的表达量降低。在盐胁迫下CkWRKY28-2和CkWRKY28-2-AS随着盐处理时间增加表达量均增加;在高温胁迫下,CkWRKY28-2和CkWRKY28-2-AS与对照相比表达量均显著下调;在低温胁迫下,CkWRKY28-2和CkWRKY28-2-AS随着处理时间增加表达量均显著下降。研究结果为CkWRKY28-2参与抗逆胁迫分子机制研究奠定了基础。Alternative splicing refers to the process in which different mRNA splice isomers are produced from an mRNA precursor by different splicing methods.WRKY transcription factors are one of the largest transcription factor families and are widely involved in growth,development,aging and abiotic stress processes.In this study,we found that WRKY28-2 from Caragana korshinskii can undergo alternative splicing and produce two different transcripts,CkWRKY28-2 and CkWRKY28-2-AS.The start codon of CkWRKY28-2 is ATG,the end codon is TGA,encoding 265 aa,the molecular weight of CkWRKY28-2 protein is 30028.18 Da,and the pI is 8.16.The start codon of CkWRKY28-2-AS is ATG,the stop codon is TGA,encoding 68 aa,the molecular weight of CkWRKY28-2-AS is 7286.71 Da,and the pI is 3.98.The results of RT-qPCR analysis showed that CkWRKY28-2 was mainly expressed in roots and stems,while CkWRKY28-2-AS was mainly expressed in leaves.Under drought stress,CkWRKY28-2 expression increased while CkWRKY28-2-AS expression decreased.Under salt stress,the expression levels of CkWRKY28-2 and CkWRKY28-2-AS increased with increasing salt treatment time.Under high temperature stress,the expression levels of CkWRKY28-2 and CkWRKY28-2-AS were significantly down-regulated compared with the control.Under low temperature stress,the expression levels of CkWRKY28-2 and CkWRKY28-2-AS decreased significantly with increasing treatment time.This study laid a foundation for the molecular mechanism of CkWRKY28-2 involved in stress resistance.

关 键 词：CkWRKY28-2 可变剪接 非生物胁迫 柠条锦鸡儿 

分 类 号：Q812[生物学—生物工程]