不同抗性遗传背景棉蚜氟啶虫胺腈及啶虫脒抗性品系转录组分析  

Transcriptome analysis of Aphis gossypii sulfoxaflor and acetamiprid-resistant strains with different genetic backgrounds of resistance

作　　者：王伟[1,2] 张仁福[1] 刘海洋[1] 丁瑞丰[1] 梁革梅[2] 姚举[1] WANG Wei;ZHANG Renfu;LIU Haiyang;DING Ruifeng;LIANG Gemei;YAO Ju(Key Laboratory of Integrated Pest Management on Crop in Northwestern Oasis,Ministry of Agriculture and Rural Affairs/Xinjiang Key Laboratory of Agricultural Biosafety/Institute of Plant Protection,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,China;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]新疆农业科学院植物保护研究所/农业农村部西北荒漠绿洲作物有害生物综合治理重点实验室/新疆农业生物安全重点实验室,乌鲁木齐830091 [2]中国农业科学院植物保护研究所/植物病虫害生物学国家重点实验室,北京100193

出　　处：《新疆农业科学》2024年第12期3078-3088,共11页Xinjiang Agricultural Sciences

基　　金：国家重点研发计划(2022YFD1400300);农业科技创新稳定支持项目(xjnkywdzc-2023004-1)。

摘　　要：【目的】研究不同抗性遗传背景棉蚜对氟啶虫胺腈和啶虫脒抗性机制差异。【方法】利用Illumina高通量测序技术,分别对2个不同抗性遗传背景棉蚜(莎车县和精河县)的田间初始品系、啶虫脒抗性品系和氟啶虫胺腈抗性品系进行转录组测序,利用生物信息学方法比较分析2个不同抗性遗传背景棉蚜种群各品系差异表达基因。【结果】莎车县氟啶虫胺腈抗性品系和啶虫脒抗性品系分别有806个和149个基因差异表达;精河县氟啶虫胺腈抗性品系和啶虫脒抗性品系与精河县的田间初始品系相比分别有233个和160个基因差异表达。在莎车县氟啶虫胺腈抗性品系和啶虫脒抗性品系中,CYP6CY59、CYP6DC1和CYP380C45均上调表达,CYP6CY12和CYP380C46均下调表达;在精河县氟啶虫胺腈抗性品系和啶虫脒抗性品系中,CYP380C46均上调表达,CYP6DC1均下调表达。此外,CYP380C45在莎车县氟啶虫胺腈抗性品系、精河县氟啶虫胺腈抗性品系和莎车县啶虫脒抗性品系中均上调表达;CYP6DC1在莎车县2个抗性品系中上调表达,但在精河县2个抗性品系中下调表达;相反,CYP380C46在精河县2个抗性品系中上调表达,但在莎车县2个抗性品系中下调表达。【结论】有多个P450基因参与棉蚜对氟啶虫胺腈和啶虫脒的抗性。相同抗性遗传背景的棉蚜氟啶虫胺腈与啶虫脒抗性品系之间差异表达的P450基因存在差异,而且不同抗性遗传背景棉蚜的氟啶虫胺腈抗性品系之间以及啶虫脒抗性品系之间差异表达的P450基因也存在差异。【Objective】To explore the differences in resistance mechanisms to sulfoxaflor and acetamiprid in cotton aphid with different genetic backgrounds of resistance.【Methods】Transcriptome sequencing by Illumina high-throughput sequencing technology was performed on initial field strain,acetamiprid-resistant strain and sulfoxaflor-resistant strain of cotton aphids with different genetic backgrounds(Yarkant and Jinghe),respectively.Meanwhile,differentially expressed genes in resistant strains of cotton aphid with different genetic backgrounds were analyzed by bioinformatics methods.【Results】By comparing the transcriptome data of sulfoxaflor-and acetamiprid-resistant strains from Yarkant and Jinghe,it was found that the sulfoxaflor-and acetamiprid-resistant strains from Yarkant had differential expression of 806 and 149 genes,respectively,and the sulfoxaflor-and acetamiprid-resistant strains from Jinghe had differential expression of 233 and 160 genes.In the sulfoxaflor-and acetamiprid-resistant strains of Yarkant,CYP6CY59,CYP6DC1,and CYP380C45 were up-regulated,but CYP6CY12 and CYP380C46 were down-regulated.In the sulfoxaflor-and acetamiprid-resistant strains of Jinghe,CYP380C46 was up-regulated whereas CYP6DC1 was down-regulated.In addition,CYP380C45 was up-regulated in Yarkant sulfoxaflor-and acetamiprid-resistant strains,and the Jinghe acetamiprid-resistant strain.CYP6DC1 was up-regulated in both Yarkant resistant strains,but down-regulated in both Jinghe resistant strains.CYP380C46 was up-regulated in both resistant strains in Jinghe but down-regulated in both resistant strains in Yarkant.【Conclusion】Several P 450 genes were involved in resistance to sulfoxaflor and acetamiprid in cotton aphids.Differences in differentially expressed P 450 genes were found between sulfoxaflor-and acetamiprid-resistant strains of cotton aphids with the same genetic background,and found between sulfoxaflor-resistant strains of cotton aphids of different genetic backgrounds,as well as between acetamiprid-resistant strain

关 键 词：棉蚜 抗性 差异表达基因 

分 类 号：S41[农业科学—植物保护]