新疆山桃腐烂病病原菌鉴定  

Identification of the pathogen of Prunus davidiana canker in Xinjiang

作　　者：唐丽 李春艳 贾文浩 刘振亚[1] 李亚鹏[1] 但红侠[1] 张王斌[1] TANG Li;LI Chunyan;JIA Wenhao;LIU Zhenya;LI Yapeng;DAN Hongxia;ZHANG Wangbin(The National Local Joint Engineering Lab of High Efficiency and Superior-Quality Cultivation and Fruit Deep Processing Technology of Characteristic Fruit Tress in Southern Xinjiang/Key Lab of Xinjiang Production and Construction Corps in Comprehensive Agricultural Pest Management in Southern Xinjiang/Scientific Observing/Experimental Station of Crops Pests in Aral,Ministry of Agriculture and Rural Affairs,Tarim University,Aral Xinjiang 843300,China;Xinjiang Applied Vocational and Technical College,Kuitun Xinjiang 833200,China)

机构地区：[1]塔里木大学/南疆特色果树高效优质栽培与深加工技术国家地方联合工程实验室/南疆农业有害生物综合治理兵团重点实验室/农业农村部阿拉尔作物有害生物科学观测实验站,新疆阿拉尔843300 [2]新疆应用职业技术学院,新疆奎屯833200

出　　处：《新疆农业科学》2024年第12期3089-3096,共8页Xinjiang Agricultural Sciences

基　　金：“新疆枣园林果树木腐烂病菌遗传多样性及致病力分化研究”(31660034)。

摘　　要：【目的】鉴定在新疆奎屯市(44°26′35″N,84°54′24″E)和阿拉尔市(40°32′30″N,81°17′35″E)的山桃腐烂枝条病症。【方法】从山桃腐烂枝条上分离出2株山桃腐烂病病原菌(KTST和TDST),测定分析田间症状、无性形态、致病性,并结合ITS、RPB2、Tef1-α多基因序列联合鉴定分析病原菌。【结果】KTST和TDST的子座结构、孔口的数量、腔室形状及孢子类型均符合壳囊孢属(Cytospora)特征,KTST与TDST分离株与GenBank中已有的Cytospora leucostoma、Cytospora chrysosperma菌株聚为一个独立的分支。将腐烂病病原菌接种在离体的健康山桃枝上,引起腐烂病症状,验证其致病性。【结论】新疆奎屯市和阿拉尔市的山桃腐烂病致病菌为Cytospora chrysosperma。【Objective】There is a typical symptom about canker disease in Prunus davidiana from Kuitun City(44°26′35″N,84°54′24″E)and Aral City(40°32′30″N,81°17′35″E),Xinjiang.With this in mind,this research aim to identify the real cause.【Methods】Pathogens(KTST and TDST)were isolated from the susceptible Prunus davidiana branches.【Results】Based on morphological characteristics,combined with ITS,RPB2 and Tef1-αmulti-genesequence analysis,Cytospora leucostoma and Cytospora chrysosperma were isolates from KTST and TDST.【Conclusion】The Koch postulates was verified by inoculating the pathogen of canker disease on the healthy branches in vitro to cause canker disease symptoms.

关 键 词：山桃腐烂病 形态特征 致病性测定 多基因联合分析 

分 类 号：S436.629[农业科学—农业昆虫与害虫防治]