一株海洋解磷菌的鉴定、促番茄幼苗生长作用及发酵培养基优化  

Identification of a Marine Phosphorus-dissolving Strain,Promotion of Tomato Seedling Growth,and Optimization of Fermentation Medium

作　　者：宋怡新 周子祺 林义利 王鸿磊[1] SONG Yixin;ZHOU Ziqi;LIN Yili;WANG Honglei(Yantai Institute of China Agricultural University,Yantai,Shandong 264670,China;Beijing Yuanmai Hill Food Co.,Ltd.,Beijing 101200,China)

机构地区：[1]中国农业大学烟台研究院,山东烟台264670 [2]北京原麦山丘食品有限公司,北京101200

出　　处：《热带农业科学》2024年第12期69-76,共8页Chinese Journal of Tropical Agriculture

基　　金：中国农业大学烟台研究院URP项目(No.U2021009)。

摘　　要：为制备一款具有解磷作用的微生物菌剂,以改善土壤养分结构,助力农业可持续发展,对一株具有解磷作用的海洋细菌DE-7进行鉴定,探究了其对番茄幼苗的促生长作用;并以菌剂的OD_(600)值为指标,通过单因素试验和响应面法,对其发酵培养基进行优化。结果表明,菌株DE-7为微小杆菌属,对番茄幼苗的植株鲜重、植株干重、根鲜重、根干重、根长和株高有促生长作用;在乳糖14.5 g/L、酵母浸粉15.0 g/L、硫酸镁5.2 g/L的培养基中,菌剂的OD_(600)值最高。To prepare a microbial agent with a phosphate-solubilizing effect,improve the soil nutrient structure,and promote sustainable agricultural development,this study identified a marine bacterium DE-7 with a phosphate-solubilizing effect.This research explored its growth-promoting effect on tomato seedlings and optimized the fermentation medium via single-factor experiments and response surface methods based on the OD_(600) value of the microbial agent.The results indicated that the bacterial strain DE-7 belongs to the Exiguobacterium sp.and has a growth-promoting effect on the fresh weight,dry weight,root fresh weight,root dry weight,root length,and height of tomato plants.The OD_(600) value of the microbial agent was the highest in the media containing lactose at 14.5 g·L^(-1),yeast extract powder at 15.0 g·L^(-1),and magnesium sulfate at 5.2 g·L^(-1).

关 键 词：微小杆菌 番茄 解磷 促生作用 响应面 

分 类 号：S154.3[农业科学—土壤学]