程序性细胞死亡因子-4对心房颤动患者外周血T淋巴细胞介导炎性反应的影响  

Effect of programmed cell death factor 4(PDCD4)on peripheral blood T lymphocyte mediated inflammatory response in patients with atrial fibrillation(AF)

作　　者：郑美娟 王姣[1] 杨玉春[1] 包针 刘琴[1] 王若楠 木胡牙提[1] ZHENG Meijuan;WANG Jiao;YANG Yuchun;BAO Zhen;LIU Qin;WANG Ruonan;Muhuyati(The First Affiliated Hospital of Xinjiang Medical University,Urumqi 830054,China)

机构地区：[1]新疆医科大学第一附属医院,乌鲁木齐830054

出　　处：《新疆医科大学学报》2025年第1期1-6,14,共7页Journal of Xinjiang Medical University

基　　金：天山英才-科技创新领军人才项目(2022TSYCLJ0065);2022年度新医大一附院“青年科研起航”专项基金—青年基金项目(2022YFY-QKQN-74)。

摘　　要：目的探讨程序性细胞死亡因子-4(PDCD4)在非瓣膜性心房颤动(NVAF)患者外周血T淋巴细胞内的表达水平变化,以及PDCD4阳性T淋巴细胞内的细胞因子的表达能力。方法选取2023年10月至2024年6月在新疆医科大学第一附属医院收治的NVAF患者70例为NVAF组,按照年龄、性别1∶1配比原则选取非心房颤动(AF)患者人群70例为对照组。收集两组研究对象体质指数(BMI)、吸烟史、饮酒史、冠心病史、2型糖尿病史、高血压史、高脂血症史、甲状腺功能亢进症史(甲亢)、慢性阻塞性肺疾病史(COPD)、阻塞性睡眠呼吸暂停病史;采集空腹肘静脉血5 mL,流式细胞术检测T淋巴细胞内PDCD4表达情况以及PDCD4阳性T淋巴细胞内细胞因子的表达情况。结果两组间年龄、性别、BMI、吸烟史、饮酒史、冠心病史、2型糖尿病史、高血压史、高脂血症史、甲亢史、慢性阻塞性肺疾病史、阻塞性睡眠呼吸暂停病史比例比较,差异无统计学意义(均P>0.05)。NVAF组患者PDCD4基因在CD4^(+)T、CD8^(+)T细胞内表达水平显著高于对照组(均P<0.001)。NVAF组患者外周血PDCD4阳性群T细胞(CD3^(+)PDCD4^(+))内白介素-4(IL-4)、白介素-17A(IL-17A)的表达水平显著高于PDCD4阴性群T细胞(CD3^(+)PDCD4^(-))(P<0.05),CD3^(+)PDCD4^(+)T细胞内干扰素-γ(IFN-γ)、肿瘤坏死因子-α(TNF-α)表达水平与CD3^(+)PDCD4^(-)T细胞群比较,差异无统计学意义(P>0.05)。结论PDCD4在NVAF患者外周血CD4^(+)T、CD8^(+)T细胞内表达上调,并且外周血PDCD4阳性群T细胞内IL-4、IL-17A的表达水平高于PDCD4阴性群T细胞,提示PDCD4可能参与了T淋巴细胞介导的炎症反应,影响AF发生发展。Objective To investigate the expression of programmed cell death factor-4(PDCD4)in periph-eral blood T lymphocytes of the patients with nonvalvular atrial fibrillation(NVAF),as well as the ex-pression levels of PDCD4^(-)positive T lymphocytes.Methods A total of 70 NVAF patients admitted to the hospital from October 2023 to June 2024 were selected for NVAF group.Additionally,70 control subjects without atrial fibrillation(AF)were matched based on age and sex at a ratio of 1∶1.Data regarding body mass index(BMI),smoking history,alcohol consumption history,coronary heart disease history,type 2 diabetes history,hypertension history,hyperlipidemia history,hyperthyroidism history,chronic obstruc-tive pulmonary disease(COPD)history and obstructive sleep apnea history were collected of both groups.5 milliliters of fasting elbow venous blood was drawn to assess PDCD4 expression in T lymphocytes and cytokine levels in PDCD4^(-)positive T lymphocytes using flow cytometry.Results No significant differences were observed between the 2 groups concerning age,sex,BMI,smoking history,drinking history,coro-nary heart disease history,type 2 diabetes history,hypertension history,hyperlipidemia history,nor re-garding histories of hyperthyroidism or COPD and obstructive sleep apnea(all P>0.05).The expression level of the PDCD4 gene in CD4^(+)T cells and CD8^(+)T cells within the NVAF group was significantly high-er than that found in control group(P<0.001).The levels of interleukin-4(IL-4)and interleukin-17A(IL-17A)in peripheral blood PDCD4^(-)positive T cells(CD3^(+)PDCD4^(+))from the NVAF group were signifi-cantly elevated compared to those in PDCD4^(-)negative T cells(CD3^(+)PDCD4^(-))(P<0.05).The expres-sion of interferon-gamma(IFN-γ)and tumor necrosis factor-alpha(TNF-α)in CD3^(+)PDCD4^(+)T cells was not statistically significant compared to the CD3^(+)PDCD4^(-)T cell population.Conclusion The upregula-tion of PDCD4 expression is the evident within peripheral blood CD4^(+)T and CD8^(+)T cells among the NVAF patients.Additiona

关 键 词：心房颤动 程序性细胞死亡因子-4 T淋巴细胞 炎性反应 

分 类 号：R541.75[医药卫生—心血管疾病]