氨氮胁迫对中华绒螯蟹生长及免疫力的影响  

Effects of Ammonia Nitrogen Stress on the Growth Performance and Immunity of Eriocheir sinensis

作　　者：罗靳 Luo Jin(Liaoning Modern Agricultural Production Base Construction Engineering Center,Shenyang 110031,Liaoning,China)

机构地区：[1]辽宁省现代农业生产基地建设工程中心,辽宁沈阳110031

出　　处：《农业技术与装备》2024年第12期128-131,134,共5页Agricultural Technology & Equipment

摘　　要：以中华绒螯蟹为研究对象,以氯化铵为养殖水体氨氮来源,设定0.00 mg/L,10.00 mg/L,20.00 mg/L,30.00 mg/L,40.00 mg/L和50.00 mg/L等6个处理,每个处理3个平行,每个平行3个重复,每个重复30只蟹,试验周期为10天。试验结束后计算中华绒螯蟹的增重率及特定生长率,并分别于胁迫试验的0天、2天、4天、6天、8天、10天测定其存活率、蜕壳率、血清SOD,CAT,LZM活性及MDA含量。结果表明:当水体氨氮质量浓度低于10 mg/L时,中华绒螯蟹增重率、特定生长率、存活率、血清SOD、CAT活性无显著变化,但河蟹长时间处于氨氮质量浓度≥10 mg/L的水体中,蜕壳率、血清LZM活性显著降低,MDA含量显著升高。综合生长及免疫指标来看,本试验条件下当水体氨氮质量浓度不超过10 mg/L时,中华绒螯蟹可正常生长,但长时间处于氨氮质量浓度≥10 mg/L的水体中,中华绒螯蟹的蜕壳率和免疫力均会受到抑制。A 10 days experiment was conducted to research the effects of different aquaculture water ammonia nitrogen level on Eriocheir sinensis growth performance and immunity.Eriocheir sinensis was the study object,and ammonium chloride was the source of the water ammonia nitrogen in this experiment.The aquaculture water ammonia nitrogen level were 0.00 mg/L,10.00 mg/L,20.00 mg/L,30.00 mg/L,40.00 mg/L,50.00 mg/L,respectly.Each handle has 3 parallels,and each parallel has 3 repeats,and there were 30 Eriocheir sinensis in each repeat.Weight gain rate and specific growth rate were computed after the stress experiment,and the survival rate,molting rate,serum SOD,CAT,LZM activity and MDA content were determined in the 0 d、2 d、4 d、6 d、8 d、10 d of the stress experiment.The results showed that Eriocheir sinensis weight gain rate,specific growth rate,survival rate,serum SOD,CAT activity had no significantly difference,when the water ammonia nitrogen level below 10 mg/L,but the molting rate,serum LZM activity reduced significantly and the MDA content increased when the Eriocheir sinensis living for a long time in water which ammonia nitrogen level was greater than or equal to 10 mg/L.Based on comprehensive growth and immune indicators,Eriocheir sinensis can growth normally when the water ammonia nitrogen below 10 mg/L,but the molting rate and immunity will be inhibited,when the Eriocheir sinensis living for a long time in water which ammonia nitrogen level was greater than or equal to 10 mg/L.

关 键 词：氨氮胁迫 中华绒螯蟹 生长 免疫力 

分 类 号：S966.16[农业科学—水产养殖]