葛根素表面分子印迹聚合物吸附性能研究及固相萃取条件优化  

Adsorption Properties of Surface Molecularly Imprinted Polymers of Puerarin and Optimization of Solid-phase Extraction Conditions

作　　者：杨海煜 苏健 罗炳有 李俊潮 钟海艺 YANG Hai-yu;SU Jian;LUO Bing-you;LI Jun-chao;ZHONG Hai-yi(College of Pharmacy,Guangxi University of Chinese Medicine,Nanning 530200,China)

机构地区：[1]广西中医药大学药学院,广西南宁530200

出　　处：《化学试剂》2025年第2期24-32,共9页Chemical Reagents

基　　金：广西自然科学基金项目(2019GXNSFBA245088);广西中医药大学一流学科建设开放课题项目(2019XK123)。

摘　　要：使用葛根素作为模板,配合丙烯酰胺(AM)作为功能单体,二甲基丙烯酸乙二醇酯(EDGMA)作为交联剂,以及偶氮二异丁腈(AIBN)作为引发剂,并添加适量的纳米二氧化硅作为表面载体,采用沉淀聚合法制备表面分子印迹聚合物(SMIPs)。吸附实验结果表明,该SMIPs对葛根素具有显著的吸附作用,在吸附液浓度为1.1 mg/mL时,吸附量可达11.9 mg/g。吸附过程符合Freundlich等温吸附模型,显示良好的吸附效果和应用潜力。材料对葛根素展现出特定的选择性。在固相萃取过程中,最优的上样、淋洗和洗脱条件分别为20%、20%和无水甲醇溶液。这项研究为从天然药物中分离、纯化和富集异黄酮类成分,特别是葛根素,提供了新颖的方法和思路。Puerarin,acrylamide(AM),ethylene glycol dimethacrylate(EDGMA),and azobisisobutyronitrile(AIBN)were used as a template,a functional monomer,a cross-linker,and an initiator,respectively.Then surface molecularly imprinted polymers(SMIPs)targeting Puerarin were successfully prepared by precipitation polymerization with adding silica nanoparticles as surface carriers.The adsorption results showed that the SMIPs had a significant adsorption for Puerarin with an adsorption amount of 11.9 mg/g when an adsorbate concentration of 1.1 mg/mL.The adsorption process followed the Langmuir and Freundlich isothermal adsorption model,which displayed a good adsorption efficiency and potential for practical application.And the SMIPs had specific selectivity to Puerarin.The optimal up-sampling,drip washing and elution conditions during the solid phase extraction were 20%,20%and anhydrous methanol solution,respectively.This study provides novel methods for an isolation,purification and enrichment of isoflavonoid components from natural medicines,especially for Puerarin.

关 键 词：葛根素 表面分子印迹 吸附 固相萃取 沉淀聚合法 

分 类 号：O631.1[理学—高分子化学]