强精片对初老雄性大鼠精子质量及雄激素生成、利用的影响  

Effects of Qiangjing Tablets on Sperm Quality,Androgen Production and Utilization in Pre-Ageing Male Rats

作　　者：严旭洪 陈帝昂[1] 尤耀东[1] 李广森[1] 张培海[1] 黄晓朋[1] 董良[1] 申毅锋 俞旭君[1,2] 常德贵 YAN Xu-hong;CHEN Di-ang;YOU Yao-dong;LI Guang-sen;ZHANG Pei-hai;HUANG Xiao-peng;DONG Liang;SHEN Yi-feng;YU Xu-jun;CHANG De-gui(TCM Regulating Metabolic Diseases Key Laboratory of Sichuan Province,Hospital of Chengdu University of Traditional Chinese Medicine,Chengdu,610072;School of Medicine and Life Sciences,Chengdu University of Traditional Chinese Medicine,Chengdu,610041)

机构地区：[1]成都中医药大学附属医院代谢性疾病中医药调控四川省重点实验室,成都610072 [2]成都中医药大学医学与生命科学学院,成都610041

出　　处：《中国中西医结合杂志》2024年第12期1482-1487,共6页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金面上项目(No.81973647);国家卫生健康委男性生殖健康重点实验室开放课题面上项目(No.2021GJP03);成都中医药大学“杏林学者”学科人才科研提升计划“优秀人才”项目(No.YXRC2020001)。

摘　　要：目的 观察强精片对初老雄性大鼠精子质量及雄激素生成、利用的调节作用,并从雄激素受体(AR)及睾酮合成通路探索其作用机制。方法 将6只2月龄SD大鼠设为对照组,将24只19月龄SD大鼠随机分为模型组、强精片低、中、高剂量组,每组6只。对照组和模型组采用生理盐水灌胃,强精片低、中、高剂量组灌胃相应剂量药物[0.72、1.44、2.88 g/(kg·d)],干预4周。检测精子密度、精子存活率、活动精子率。ELISA法检测血清黄体生成素(LH)、总睾酮(TT)、游离睾酮(FT)及性激素结合球蛋白(SHBG)水平,并计算睾酮分泌指数(TSI)。Western Blot法检测睾丸AR、类固醇急性生成调节蛋白(StAR)、细胞色素P450侧链裂解酶(CYP11A1)、细胞色素P450 17-α-羟化酶/20-裂解酶(CYP17A1)、3β-羟基类固醇脱氢酶(3β-HSD)蛋白表达水平。结果 与对照组比较,模型组精子密度、精子存活率、活动精子率、TSI降低(P<0.01),血清SHBG水平及AR、StAR、3β-HSD蛋白表达升高(P<0.05,P<0.01)。与模型组比较,强精片高剂量组精子密度、精子存活率、活动精子率升高(P<0.01);强精片中、高剂量组TSI降低(P<0.05),CYP17A1蛋白表达升高(P<0.01);强精片低、中、高剂量组AR蛋白表达升高(P<0.01),StAR、3β-HSD、CYP11A1蛋白表达降低(P<0.01)。结论 强精片能改善初老雄性大鼠精液质量,缓解睾酮生成过程中的代偿,增加睾丸组织雄激素利用,其机制可能与调控StAR、3β-HSD、CYP11A1、CYP17A1并上调睾丸组织AR表达有关。Objective To observe the effects of Qiangjing Tablets(QJT)on sperm quality and the production and utilization of androgens in pre-ageing male rats,and to explore the possible mechanisms through the androgen receptor(AR)and testosterone synthesis pathways.Methods Six 2-month-old SD rats were set as the control group,and 2419-month-old SD rats were randomly divided into model group,QJT low,medium and high dose group,with 6 rats in each group.The control group and the model group were treated with normal saline by gavage,and the QJT low,medium and high dose group were treated with corresponding doses of QJT(0.72,1.44,2.88 g·kg^(-1)·d^(-1),respectively).The intervention lasted for 4 weeks.Sperm density,sperm viability and motile sperm rate were measured.Serum luteinizing hormone(LH),total testosterone(TT),free testosterone(FT),and sex hormone-binding globulin(SHBG)were measured by ELISA,and testosterone secretion index(TSI)was calculated.Protein expression of testis AR,steroid acute production regulatory protein(StAR),cytochrome P450 side chain lyase(CYP11A1),cytochrome P45017-α-hydroxylase/20-lyase(CYP17A1)and 3β-hydroxy steroid dehydrogenase(3β-HSD)were detected by Western Blot.Results Compared with the control group,the sperm density,sperm viability,sperm motility rate and TSI decreased(P<0.01),while serum SHBG and the expression of testis AR,StAR and 3β-HSD increased(P<0.05,P<0.01)in the model group.Compared with the model group,the sperm density,sperm viability and motile sperm rate increased in QJT high dose group(P<0.01).TSI decreased(P<0.05),expression of testis CYP17AR increased in QJT high and medium dose group(P<0.01).The expression of testis AR increased(P<0.01),the expression of testis StAR,3β-HSD and CYP11A1 protein decreased(P<0.01)in the QJT low,medium,and high dose group.Conclusion QJT could improve sperm quality in pre-ageing male rats,alleviate the compensation of testosterone production and increase the utilization of androgen in the testis of pre-ageing male rats,which may be related to th

关 键 词：强精片 初老 生殖衰老 精子 雄激素 中药复方 

分 类 号：R285.5[医药卫生—中药学]