具有序列选择性的长寿命β-淀粉样蛋白荧光探针研究  

Long-lived beta-amyloid fluorescent probes with sequence selectivity

作　　者：曹慧芳[1] 陈妍[2] 于会娟[2] CAO Huifang;CHENYan;YU Huijuan(The Second Affiliated Hospital of Luohe Medical College,Luohe 462300,China;School of Chemical Engineering and Light Industry,Guangdong University of Technology,Guangzhou 510006,China)

机构地区：[1]漯河医学高等专科学校第二附属医院,河南漯河462300 [2]广东工业大学轻工化工学院,广东广州510006

出　　处：《广东药科大学学报》2025年第1期26-32,共7页Journal of Guangdong Pharmaceutical University

基　　金：国家自然科学基金项目(21907016)。

摘　　要：目的开发一款可检测所有Aβ聚集体,并具有序列选择性的长寿命Aβ荧光探针(Ru-itatp)。方法利用荧光光谱测试Ru-itatp与Aβ蛋白的作用及荧光检测效果,利用MTT法测试生物毒性,利用激光共聚焦显微成像研究细胞及组织成像效果。结果Ru-itatp对Aβ_(1-42)蛋白有灵敏的荧光强度和荧光寿命响应,可与不同聚集形态(单体、低聚体、纤维体)Aβ结合,能特异性标记大脑中的Aβ斑块,并具有较低的生物毒性。结论Ru-itatp具有较强的Aβ蛋白识别能力及较低的生物毒性,有望成为阿尔茨海默症早期诊断和治疗的有力工具。Objective To develop a highly selective Aβfluorescent probe that can detect all Aβaggregated species with sequence recognition.Methods The interaction of Ru-itatp with Aβprotein and fluorescence detection were tested by fluorescence spectroscopy,its biotoxicity was tested by MTT method,and its cellular and tissue imaging effect was investigated by laser confocal microimaging.Results Ru-itatp had a sensitive fluorescence intensity and fluorescence lifetime response to Aβ_(1-42)proteins,binded to Aβin different aggregated forms(monomers,oligomers,and fibrils),and specifically labeled Aβplaques in the brain with low biotoxicity.Conclusion Ru-itatp,with its strong Aβprotein recognition ability and low biotoxicity,is expected to be a powerful tool for early diagnosis and treatment of Alzheimer’s disease.

关 键 词：阿尔茨海默症 Β-淀粉样蛋白 荧光探针 

分 类 号：R981[医药卫生—药品]