益气聪明汤加减方防治药源性及老年性耳聋的作用及机制初探  

Pharmacological effects and mechanisms of modified Yiqi Congming Decoction in the prevention and treatment of drug-induced and age-related hearing loss

作　　者：陈伟强[1] 杨艾霖 林粤圣 余冲 袁旭江[1] 温露 张明 陈钢[1] CHEN Weiqiang;YANG Ailin;LIN Yuesheng;YU Chong;YUAN Xujiang;WEN Lu;ZHANG Ming;CHEN Gang(Guangdong Provincial Key Laboratory for Research and Evaluation of Pharmaceutical Preparations,Center for Drug Research and Development,Guangdong Pharmaceutical University,Guangzhou 510006,China;School of Pharmacy,Guangdong Pharmaceutical University,Guangzhou 510006,China;Guangdong Sunho Pharmaceutical Co.Ltd,Zhongshan 528437,China)

机构地区：[1]广东药科大学新药研发中心/广东省药物制剂研究与评价重点实验室,广东广州510006 [2]广东药科大学药学院,广东广州510006 [3]广东星昊药业有限公司,广东中山528437

出　　处：《广东药科大学学报》2025年第1期99-107,共9页Journal of Guangdong Pharmaceutical University

基　　金：国家自然科学基金项目(82474092);广州市重点研发计划项目(2023B03J1293);粤澳科技创新联合资助项目(2022A0505020014);广东药科大学大学生创新训练计划立项项目(S202410573005X,202410573007X)。

摘　　要：目的 探究益气聪明汤加减方对药源性及老年性耳聋的防治作用并初步探索其作用机制。方法 采用斑马鱼模型,利用卡那霉素、顺铂和过氧化氢(H2O2)建立毛细胞损伤模型,统计毛细胞荧光面积来评价益气聪明汤加减方的毛细胞保护作用。利用豚鼠动物模型,通过卡那霉素、顺铂和D-半乳糖建立豚鼠耳聋模型,检测听力阈值变化和观察耳蜗基底膜毛细胞来评价益气聪明汤加减方的防治作用。通过网络药理学初步探究其作用机制。结果 斑马鱼模型中,益气聪明汤加减方质量浓度在1.0~2.0 mg/mL时,能显著降低卡那霉素、顺铂和H2O2诱导的毛细胞损伤(P<0.05,P<0.01);豚鼠模型中,益气聪明汤加减方高剂量组能显著降低卡那霉素、顺铂和D-半乳糖诱导的听力损失(P<0.01),低剂量组能显著降低D-半乳糖诱导的听力损失(P<0.01);网络药理学筛选出5个主要活性成分、5个核心靶点、915个GO条目、153条KEGG通路,包括AGE-RAGE信号通路、MAPK信号通路等。结论 益气聪明汤加减方对药源性及老年性耳聋能起到明显的防治作用,其机制可能与调控AGE-RAGE信号通路、MAPK信号通路等相关。Objective To investigate the preventive and therapeutic effects and mechanisms of the modified Yiqi Congming decoction on drug-induced and age-related hearing loss.Methods A zebrafish model was employed to establish a hair cell damage model induced by kanamycin,cisplatin,and hydrogen peroxide.The protective effect of modified Yiqi Congming decoction on hair cells was evaluated by quantifying the fluorescent area of the hair cells.A guinea pig model of hearing loss was established by using kanamycin,cisplatin,and D-galactose.The effects of Yiqi Congming decoction were evaluated by detecting changes in hearing threshold and observing hair cells in the cochlear basement membrane.The mechanisms were preliminarily explored through network pharmacology.Results In zebrafish model,the decoction significantly reduced hair cell damage induced by kanamycin,cisplatin,and hydrogen peroxide at concentrations of 1.0~2.0 mg/mL(P<0.05,P<0.01).In the guinea pig model,the high-dose group of the decoction significantly alleviated hearing loss induced by kanamycin,cisplatin,and D-galactose(P<0.01),while the low-dose group notably reduced D-galactose-induced hearing loss (P<0.01). Network pharmacology identified five main active components, five core targets, 915 GO entries, and153 KEGG pathways, including the AGE-RAGE signaling pathway and the MAPK signaling pathway. ConclusionModified Yiqi Congming decoction exhibits significant preventive and therapeutic effects on drug-induced andage-related hearing loss, potentially through modulating pathways such as AGE-RAGE and MAPK signaling.

关 键 词：益气聪明汤加减方 药源性耳聋 老年性耳聋 网络药理学 

分 类 号：R965[医药卫生—药理学]