家蚕微粒子病分子检测技术研究进展  

Research Progress on Molecular Detection Techniques for Silkworm Pébrine

作　　者：孟宪志 房德文 郭钊义 于弋翔 周泽扬[1,2,6] 孙学强 潘国庆[1,2] MENG Xianzhi;FANG Dewen;GUO Zhaoyi;YU Yixiang;ZHOU Zeyang;SUN Xueqiang;PAN Guoqing(State Key Laboratory of Resource Insects,Southwest University,Chongqing 400715,China;Chongqing Key Laboratory of Microsporidia Infection and Control,Southwest University,Chongqing 400715,China;Shandong Guangtong Silkworm Seed Group Co.,Ltd.,Qingzhou Shandong 262500,China;Shandong Province Lusi Silkworm Seed Quality Inspection Institute Co.,Ltd.,Qingzhou Shandong 262500,China;Qingdao Lijian Biotechnology Co.,Ltd.,Qingdao Shandong 266000,China;College of Life Science,Chongqing Normal University,Chongqing 401331,China)

机构地区：[1]资源昆虫高效养殖与利用全国重点实验(西南大学),重庆400715 [2]西南大学微孢子虫感染与防控重庆市重点实验室,重庆400715 [3]山东广通蚕种有限公司,山东青州262500 [4]山东省鲁丝桑蚕种质量检验所有限公司,山东青州262500 [5]青岛立见生物科技有限公司,山东青岛266000 [6]重庆师范大学生命科学学院,重庆401331

出　　处：《蚕学通讯》2024年第4期42-48,共7页Newsletter of Sericultural Science

基　　金：重庆市自然科学基金项目(cstc2021jcyj-msxmX1003);重庆市自然科学基金创新群体项目(cstc2021jcyj-cxtt0005);山东广通蚕种有限公司横向项目(M2024005);青岛立见生物科技有限公司横向项目(F2023583)。

摘　　要：家蚕微粒子病(Pébrine)由家蚕微孢子虫(Nosema bombycis)感染导致,是蚕业生产中的重大疫病。N.bombycis不仅能够在家蚕个体间水平传播,还能通过胚胎垂直传播,给防控工作带来极大挑战。N.bombycis是蚕业生产中唯一的法定检疫对象,目前行业内普遍采用的检测方法是19世纪巴斯德创建的母蛾显微镜检查法,随后发展为针对集团母蛾、蚕种和蚁蚕的显微镜检查,并配套制定了相应的诊断标准。随着现代分子生物学技术的进步,N.bombycis的检测方法得到了显著发展,相继报道了核酸检测、抗原检测、PCR-ELISA、核酸侧向层析试纸条以及基于核酸扩增和蛋白质识别的新型电化学检测等方法。本文综述了以上检测方法的研究进展,并探讨其应用场景,以期为家蚕微粒子病分子检测体系的构建提供参考。Pébrine,caused by Nosema bombycis,is a significant threat to sericulture.N.bombycis spreads both horizontally between silkworms and vertically through embryos,presenting considerable challenges for effective prevention and control.As the only legally mandated quarantine pathogen in sericulture,N.bombycis is commonly detected using the moth microscopy method developed by Pasteur in the 19th century.This method has since evolved to include group moth,silkworm seed,and larvae microscopy examinations,with standardized diagnostic protocols.In recent years,advancements in molecular biology techniques have greatly improved the detection methods for larvae.New approaches such as nucleic acid detection,antigen detection,PCR-ELISA,nucleic acid lateral flow assay strips,and novel electrochemical detection based on nucleic acid amplification and protein recognition have been reported.This review summarizes the research progress of these detection methods and explores their application scenarios,aiming to provide valuable insights for establishing a comprehensive molecular detection system for pébrine.

关 键 词：家蚕微粒子病 家蚕微孢子虫 分子检测 核酸检测 抗原检测 新型电化学检测 

分 类 号：S85[农业科学—兽医学]