5株家蚕微孢子虫单克隆抗体的抗原鉴定  

Antigen Identification of Five Monoclonal Antibodies against Nosema bombycis

作　　者：马成[1,2,3] 杨东林 陈洁 潘国庆[1,2] 李田[1,2] 周泽扬[1,2,5] MA Cheng;YANG Donglin;CHEN Jie;PAN Guoqing;LI Tian;ZHOU Zeyang(State key laboratory of Resource Insects,Southwest University,Chongqing 400715,China;Chongqing Key Laboratory of Microsporidia Infection and Control,Southwest University,Chongqing,400715,China;Bishan middle school,Chongqing 402760,China;Chongqing University of Arts and Sciences,Chongqing 402160,China;College of Life Sciences,Chongqing Normal University,Chongqing 401331,China)

机构地区：[1]资源昆虫高效养殖与利用全国重点实验室(西南大学),重庆400715 [2]西南大学微孢子虫感染与防控重庆市重点实验室,重庆400715 [3]重庆市璧山中学校,重庆402760 [4]重庆文理学院药学院,重庆402160 [5]重庆师范大学生命科学学院,重庆401331

出　　处：《蚕学通讯》2024年第4期95-99,共5页Newsletter of Sericultural Science

基　　金：国家自然科学基金重点项目(30930067);重庆市自然科学基金创新群体项目(cstc2021jcyj-cxtt0005);重庆市现代农业产业技术体系(COMAITS202311);中央高校基本科研业务费项目(SWU-KT22033,SWU-XDJH202322)。

摘　　要：家蚕微孢子虫(Nosema bombycis)是引起家蚕微粒子病的病原。通过检测筛查带病蚕蛾或蚕卵以减少家蚕微孢子虫垂直传播对蚕种生产和家蚕养殖造成的危害,是防控微粒子病的主要方法。孢壁蛋白是家蚕微孢子虫孢壁结构组成的重要成分,且具有种属特异性,是病原检测的主要靶标。本研究选取家蚕微孢子虫孢子发芽液制备的5株单克隆抗体,采用孢子体外发芽、孢壁蛋白原核表达及免疫印迹分析等方法,明确了5株单抗的抗原蛋白均为高丰度的孢壁蛋白NbSWP1。研究结果为利用NbSWP1作为免疫学检测靶标进行快速检测试纸条的研制提供了数据基础。Nosema bombycis is the causative agent of pébrine disease in silkworms.To prevent and control pébrine disease,the primary strategy involves reducing the impact of vertical transmission of N.bombycis on silkworm egg production and breeding is detection and screening of infected silkworm moths or eggs.Spore wall proteins,which exhibit species-specific characteristics,are crucial components of the spore wall structure of N.bombycis and serve as key targets for detection.In this study,five monoclonal antibodies prepared from the spore germination fluid of N.bombycis were selected.By using methods such as spore germination in vitro,prokaryotic expression of spore wall protein,and immunoblotting analysis,it was clarified that the antigenic proteins recognized by the five monoclonal antibodies were the high-abundance spore wall protein SWP1.This research provides a foundational dataset for the development of rapid detection test strips utilizing SWP1 as an immunological detection target.

关 键 词：家蚕微孢子虫 孢壁蛋白1 单克隆抗体 抗原鉴定 

分 类 号：S85[农业科学—兽医学]