番茄黄化曲叶病毒胁迫下外源NO对番茄抗氧化物酶基因表达的影响  

Effect of Exogenous NO on Expression of Tomato Antioxidant Enzyme Gene Under Tomato Yellowing Leaf Curl Virus Stress

作　　者：罗金城 朱晓林 魏小红[1] 王贤 王宝强[1] 杜雪芬 LUO Jincheng;ZHU Xiaolin;WEI Xiaohong;WANG Xian;WANG Baoqiang;DU Xuefen(Gansu Provicial Key Lab of Aridland Crop Science,Gansu Key Lab of Crop Genetic&Germplasm Enhancement,College of Life Science and Technology,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃省作物遗传改良与种质创新重点实验室,甘肃省干旱生境作物学重点实验室,兰州730070

出　　处：《中国农业科技导报(中英文)》2025年第2期125-135,共11页Journal of Agricultural Science and Technology

基　　金：国家自然科学基金项目(32060401)。

摘　　要：为探究番茄黄化曲叶病毒(tomato yellow leaf curl virus,TYLCV)胁迫下外源一氧化碳(nitric oxide,NO)对番茄抗氧化物酶基因表达的影响,以易感病番茄品种金鹏1号为试验材料,在对照(CK)、TYLCV(TY)和NO+TYLCV(NO+TY)3种处理下,通过转录组测序、荧光qRT-PCR和生物信息学分析进行研究。结果表明,在番茄基因组中共筛选出55个抗氧化酶相关编码基因,其中存在于不同亚细胞区室中含有外显子数目最多的抗氧化酶基因均显著响应TYLCV胁迫。NO介导的抗氧化酶编码基因数量在不同亚细胞区室的分布表现为叶绿体>细胞膜>细胞质>过氧化物酶体>液泡,其中Chl Cu-Zn SOD、Chl MR2、Chl GR、Per MR、Pla CAT1和Pla CAT7的表达量显著上调;Chl Fe SOD1、Chl Fe SOD2、Cyt GPX、Cyt APX1、Cyt APX2 L-5、Pla CAT3、Pla CAT8和Vac CAT的表达量显著下调。实时荧光qRT-PCR验证发现,Chl GR、Min Mn SOD和Per CAT2响应TYLCV表达,Chl Cu-Zn SOD、Pla CAT7、Pla CAT8和Cyt APX2 L-5响应TYLCV和NO表达。以上结果为研究NO在提高番茄抗病性机制中的作用提供理论依据。In order to investigate the effect of exogenous nitric oxide(NO)on the expression of tomato antioxidant gene under the stress of tomato yellow leaf curl virus(TYLCV),the susceptible tomato Jinpeng 1 was used as the experimental material.Transcriptome sequencing,fluorescent qRT-PCR and bioinformatics analysis were performed under control(CK),TYLCV(TY)and NO+TYLCV(NO+TY)treatments.The results showed that a total of 55 antioxidase-coding genes were selected in the tomato genome,among which the antioxidase-containing genes with the highest number of exons in different subcellular regions were significantly responsive to TYLCV stress.The number of NO-mediated antioxidant enzyme coding genes in different subcellular compartms showed chloroplast>cell membrane>cytoplasm>peroxisome>vacuole,in which the expression levels of Chl Cu-Zn SOD,Chl MR2,Chl GR,Per MR,Pla CAT1 and Pla CAT7 were significantly up-regulated,and the expression levels of Chl Fe SOD1,Chl Fe SOD2,Cyt GPX,Cyt APX1,Cyt APX2 L-5,Pla CAT3,Pla CAT8 and Vac CAT were significantly down-regulated.Fluorescence qRT-PCR verified that Chl GR,Min Mn SOD and Per CAT2 responded to TYLCV,and Chl Cu-Zn SOD,Pla CAT7,Pla CAT8 and Cyt APX2 L-5 responded to TYLCV and NO.Above results provided theoretical basis for studying the mechanism of NO in improving tomato disease resistance.

关 键 词：番茄 番茄黄花曲叶病 一氧化氮 抗氧化酶基因 

分 类 号：S641.2[农业科学—蔬菜学] Q344[农业科学—园艺学]