苏云金芽孢杆菌对茶尺蠖肠道细菌多样性的影响  

Effects of Bacillus thuringiensi on Intestinal Bacteria in Ectropis obliqua

作　　者：金若珩 李晓宇 姚经武 王蓓蓓 曹春霞 黄大野 JIN Ruoheng;LI Xiaoyu;YAO Jingwu;WANG Beibei;CAO Chunxia;HUANG Daye(Hubei Biopesticide Engineering Research Centre,Wuhan 430064,China)

机构地区：[1]湖北省生物农药工程研究中心,武汉430064

出　　处：《中国农业科技导报(中英文)》2025年第2期141-149,共9页Journal of Agricultural Science and Technology

基　　金：湖北省重点研发计划项目(2022BBA0078);恩施州科技计划项目(D20220039);湖北省农业科技创新中心创新团队项目(2021-620-000-001-027)。

摘　　要：茶尺蠖(Ectropis obliqua)是茶叶上的重要害虫,给茶叶产业带来严重经济损失。苏云金芽孢杆菌(Bacillus thuringiensis,Bt)作为防治茶尺蠖的关键生物杀虫剂,在茶尺蠖的绿色防控中发挥重要作用。为明确Bt处理对茶尺蠖幼虫肠道菌群的影响,基于Illumina平台,采用16S rDNA高通量测序技术对不同水平Bt悬浮液处理的茶尺蠖幼虫肠道细菌的群落结构和种群多样性进行比较分析。结果表明,使用低剂量Bt处理茶尺蠖幼虫后,其肠道细菌组成与对照处理相比并未发生明显变化;但在较高剂量Bt处理下,茶尺蠖幼虫肠道细菌的群落多样性及丰度显著增加,表明茶尺蠖幼虫肠道细菌群落参与了对Bt侵染的响应。以上研究结果为深入探究Bt杀虫机制提供依据,并为提高Bt杀虫毒力提供新的思路。Ectropis obliqua is one of the most important pests on tea,which has brought serious economic losses to the tea industry.Bacillus thuringiensis(Bt),as a key biological insecticide for the control of E.obliqua,plays an important role in the green prevention and control of diseases and pests in tea gardens.To clarify the effect of Bt treatment on intestinal bacteria of E.obliqua larvae,the Illumina platform using 16S rDNA high-throughput sequencing was applied to compare the structure and population diversity of intestinal commensal community in E.obliqua larvae treated with different dosage of Bt suspensions.The results showed that,compared with CK treatment,the intestinal bacterial composition of E.obliqua larvae treated with lower dosage of Bt did not change significantly,but those treated with higher dosage of Bt had significant effect on the composition of intestinal bacteria in E.obliqua larvae.And the treatments with higher dosage of Bt significantly increased the diversity and abundance of intestinal bacteria in E.obliqua larvae,which indicated that intestinal microorganisms were involved in the response of E.obliqua larvae to Bt infection.Above results provided basis for further exploring the insecticidal mechanism of Bt,and provided new ideas for improving the virulence of Bt.

关 键 词：茶尺蠖 苏云金芽孢杆菌 生物防治 肠道细菌 16S rDNA 

分 类 号：S476[农业科学—农业昆虫与害虫防治]