大豆IGT基因家族的全基因组鉴定及组织表达分析  

Genome-wide identification and tissue expression analysis of IGT gene family in soybean

作　　者：陈梦瑶 胡怡然 郑志富[1] 潘天 CHEN Mengyao;HU Yiran;ZHENG Zhifu;PAN Tian(College of Advanced Agricultural Sciences,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)

机构地区：[1]浙江农林大学现代农学院,浙江杭州311300

出　　处：《浙江农林大学学报》2025年第1期64-73,共10页Journal of Zhejiang A&F University

基　　金：浙江省自然科学基金资助项目(LQ24C130001);浙江农林大学人才启动基金资助项目(2022LFR109)。

摘　　要：【目的】利用生物信息学方法对大豆‘天隆1号’Glycine max ‘Tianlong 1’ IGT基因家族进行全基因组鉴定,探究IGT基因家族在大豆中的潜在功能。【方法】通过对大豆‘天隆1号’IGT蛋白结构域进行BLASTP搜索,确定GmIGTs;以生物信息学方法分析其进化关系、基因结构、保守基序、顺式作用元件、共线性关系,以及在不同组织部位表达模式。【结果】共鉴定出17个GmIGT基因,并根据其系统发育关系将其分为4个分支:TAC、IGT-like、DRO和LAZY。蛋白质保守基序分析发现:‘天隆1号’IGT蛋白均含有Motif2。染色体定位和共线性分析显示:GmIGT不均匀地定位在11条染色体上,并且片段复制可能在GmIGT基因的扩张中发挥了重要作用。顺式作用元件分析表明:GmIGT的表达可能与光响应、生理响应、植物激素响应和胁迫等相关。此外,实时荧光定量聚合酶链式反应分析表明:GmIGT基因具有明显的组织特异表达特点,GmIGT4在所有组织中表达量都相对较高,GmIGT4、GmIGT10与GmIGT17在茎与叶柄中高度表达。【结论】GmIGT基因可能在塑造大豆‘天隆1号’株型中发挥某些潜在作用,GmIGT4、GmIGT10与GmIGT17可能为该过程中的核心基因。[Objective]This study aims to employ bioinformatics methods to perform a comprehensive genome-wide identification of the soybean IGT gene family and explore the potential functions of IGT gene family in soybean.[Method]Soybean cultivar Glycine max‘Tianlong 1’was selected and GmIGTs were identified by BLASTP search of IGT protein domain.The evolutionary relationships,gene structure,conserved motifs,cis-acting elements,and collinearity relationships were analyzed by bioinformatics methods.The expression patterns of GmIGTs in different tissue parts were analyzed.[Result]A total of 17 GmIGT genes were identified and classified into 4 branches based on their phylogenetic relationships:TAC,IGT-like,DRO,and LAZY.Protein conserved motif analysis revealed that all IGT proteins contained Motif2.Chromosomal localization and collinearity analysis showed that GmIGT genes were unevenly distributed on 11 chromosomes,and segmental duplication might have played a significant role in the expansion of GmIGT gene.Cis-acting element analysis indicated that GmIGT expression might be associated with light response,physiological response,plant hormone response and stress.RT-qPCR analysis demonstrated that GmIGT gene had obvious tissue-specific expression characteristics.Among them,GmIGT5 exhibited relatively high expression levels in all tissues,while GmIGT4 and GmIGT10 were highly expressed in stems and petioles.[Conclusion]GmIGT gene may play a potential role in shaping soybean plant architecture,and GmIGT4 and GmIGT10 may be the core genes involved in this process.

关 键 词：大豆‘天隆1号’ 株型 IGT基因家族 表达模式分析 

分 类 号：S565.1[农业科学—作物学]