雌性榧树种实性状和SSR标记的遗传变异  

Genetic variation of seed traits and SSR markers in female Torreya grandis

作　　者：李柯豫 陈荣[1] 刘琏 蔡晓郡 姜郑楚 谢前丹 俞晨良 喻卫武[1] LI Keyu;CHEN Rong;LIU Lian;CAI Xiaojun;JIANG Zhengchu;XIE Qiandan;YU Cheniang;YU Weiwu(State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China;Fuyang Agriculture and Rural Bureau of Hangzhou City,Hangzhou 311499,Zhejiang,China)

机构地区：[1]浙江农林大学省部共建亚热带森林培育国家重点实验室,浙江杭州311300 [2]浙江省杭州市富阳区农业农村局,浙江杭州311499

出　　处：《浙江农林大学学报》2025年第1期94-102,共9页Journal of Zhejiang A&F University

基　　金：浙江省果品育种重大专项(2021C02066-11)。

摘　　要：【目的】探究不同种群雌性榧树Torreya grandis种实性状,基于简单重复序列标记(SSR)引物对榧树的种群遗传变异进行分析,比较榧树种群间、种群内种实表型、品质及遗传多样性的差异。【方法】以浙江富阳、嵊州、临安、建德及安徽黄山种群雌性榧树为材料,通过方差分析、主成分分析对榧树叶片及种实表型进行比较;以浙江富阳、嵊州、临安、淳安及安徽黄山种群雌性榧树为材料,通过SSR分子标记对榧树遗传多样性进行比较。【结果】榧树种实分析发现:叶质量、叶形指数、种实质量、种形指数、种核质量、核形指数、假种皮厚、种壳厚等8个指标在种群间和种群内个体差异极显著(P<0.01);脂肪相对含量及可溶性糖质量分数在种群间差异极显著(P<0.01),脂肪相对含量为29.36%~42.35%,榧树种实外观和种仁品质变异丰富。SSR引物分析发现:榧树种群的Nei’s遗传多样性指数(H)均值为0.400,Shannon’s信息指数(I)均值为0.650,多态性信息含量(P_(IC))均值为0.400。淳安种群的遗传多样性(H=0.410,I=0.658)最高,嵊州种群(H=0.369,I=0.565)最低。92%总遗传变异存在于种群内。【结论】榧树种实的表型、品质在种群间和种群内存在遗传变异,淳安种群遗传多样性最高,且种群内遗传变异大于种群间。[Objective]This study is to investigate the seed traits of female Torreya grandis,analyze the genetic variation of the population based on SSR primers,and compare the differences in seed phenotype,quality,and genetic diversity between and within populations of T.grandis.[Method]Female T.grandis from Fuyang,Shengzhou,Lin’an,Jiande in Zhejiang Province and Mount Huangshan in Anhui Province were used as materials to compare the phenotypic traits of T.grandis leaves and seeds through variance analysis and principal component analysis.The genetic diversity of T.grandis was compared by SSR molecular markers using female T.grandis from Fuyang,Shengzhou,Lin’an,Chun’an and Mount Huangshan as raw materials.[Result]Analysis of seed traits revealed that there were significant individual differences(P<0.01)in 8 indicators:leaf quality,leaf shape index,seed mass,seed shape index,seed weight,kernel shape index,seed coat thickness,and shell thickness between and within populations.The relative fat content and soluble sugar content also showed extremely significant differences among populations(P<0.01),with fat content ranging from 29.36%to 42.35%,indicating rich variation in T.grandis seed appearance and kernel quality.SSR primer analysis showed that the mean Nei’s genetic diversity index(H)was 0.400,the mean Shannon’s information index(I)was 0.650,and the mean polymorphic information content(PIC)was 0.400.Chun’an population exhibited the highest genetic diversity(H=0.410,I=0.658),while Shengzhou population showed the lowest(H=0.369,I=0.565).92%of the total genetic variation existed within the population.[Conclusion]There are genetic variations in the phenotype and quality of T.grandis between and within populations.The genetic diversity of Chun’an population is the highest,and the genetic variation within the population is greater than that between populations.

关 键 词：榧树 不饱和脂肪酸 可溶性糖 SSR标记 遗传变异 

分 类 号：S722.3[农业科学—林木遗传育种]