植物钙信号指示剂的研究进展  

Research progress on plant calcium signaling indicators

作　　者：陆雯艳 刘金枝 沈钰琪 亓果宁 柳参奎 任慧敏 LU Wenyan;LIU Jinzhi;SHEN Yuqi;QI Guoning;LIU Shenkui;REN Huimin(College of Forestry and Biotechnology/State Key Laboratory of Subtropical Silviculture,Zhejiang A&F University,Hangzhou 311300,Zhejiang,China)

机构地区：[1]浙江农林大学林业与生物技术学院/省部共建亚热带森林培育国家重点实验室,浙江杭州311300

出　　处：《浙江农林大学学报》2025年第1期202-209,共8页Journal of Zhejiang A&F University

基　　金：省部共建亚热带森林培育国家重点实验室开放基金(SKLSS-KF2022-08);国家重点研发计划青年科学家项目(2021YFD2200900)。

摘　　要：钙离子(Ca^(2+))是植物信号传递中重要的第二信使,对植物的生长发育和胁迫响应起着至关重要的作用。近年来,随着生物化学和分子生物学技术的不断进步,钙信号指示剂在植物研究中的应用取得了显著进展。本文综述了关于钙信号指示剂在植物应用中的发展情况,包括钙指示剂的分类、钙信号检测原理及其在植物应用中的发展。钙信号指示剂的应用提供了可视化观察植物细胞内钙离子动态变化的有效手段,将植物细胞内的钙离子浓度转化为荧光信号。随着指示剂的发展,从最早的化学荧光指示剂逐步向基因编码钙指示剂过渡,能更加精确、实时、生物友好地观察到植物钙信号,对植物应对外界刺激时分子水平的应激手段有了更加深入的认识,成为研究植物应激分子水平上的重要生理指标。同时,本文还对钙信号指示剂在植物研究中存在的挑战和未来发展方向进行了讨论,指出了植物钙信号研究的特殊性与植物细胞中孵育钙指示剂的挑战性,以期为进一步推动该领域的研究提供参考和启示。Calcium ion(Ca^(2+))is the important second messenger that plays a crucial role in plant growth and development and stress response.In recent years,with the continuous progress of biochemistry and molecular biology technology,the application of calcium signaling indicators in plant research has made remarkable progress.This paper reviewed the recent developments on calcium signal indicators in plant applications,including the classification of calcium indicators,the principles of calcium signal detection and their development in plant applications.The application of calcium signal indicators provide an effective means to visualize and observe the dynamic changes of calcium ion in plant cells,converting calcium concentrations in plant cells into fluorescent signals.With the development of indicators,gradual transition changing from the earliest chemical fluorescent indicators to genetically encoded calcium indicators,calcium signals can be observed more accurate,real-time and biologically friendly in plant cells,and have a more in-depth understanding of the means of stress at the molecular level when the plant responds to external stimuli,and became an important physiological indicator at the molecular level of the study of plant stress.Meanwhile,the challenges and future development directions of calcium signaling indicators in plant research were discussed,pointing out the specificity of plant calcium signaling research with the challenges of incubating calcium indicators in plant cells,with a view to providing references and insights to further promote research in this field.

关 键 词：钙离子 钙信号指示剂 植物 生长发育 胁迫响应 

分 类 号：Q945[生物学—植物学]