臭氧氧化-粉末活性炭-超滤对污水厂二级出水中抗生素抗性基因的去除效能及影响因素  

Removal Efficiency and Influencing Factors of Antibiotic Resistance Genes in Secondary Effluent by the Process of O 3-PAC-UF

作　　者：孙丽华[1] 邓斯 史鹏飞 王春芳 SUN Li-hua;DENG Si;SHI Peng-fei;WANG Chun-fang(Key Laboratory of Urban Rainwater System and Water Environment Ministry of Education,Beijing University of Civil Engineering and Architecture,Beijing 100044,China;School of Environmental and Energy Engineering,Beijing University of Civil Engineering and Architecture,Beijing 100044,China;Beijing General Municipal Engineering Design&Research Institute Co.,Ltd.,Beijing 100082,China)

机构地区：[1]北京建筑大学城市,雨水系统与水环境教育部重点实验室,北京100044 [2]北京建筑大学环境与能源工程学院,北京100044 [3]北京市市政工程设计研究总院有限公司,北京100082

出　　处：《科学技术与工程》2025年第3期1298-1305,共8页Science Technology and Engineering

基　　金：国家自然科学基金(52370004,52070011);北京建筑大学研究生创新项目(PG2024085)。

摘　　要：抗生素抗性基因(antibiotic resistance genes,ARGs)作为新兴环境污染物已对人类健康造成威胁,有研究表明,污水处理厂的二级出水是环境中ARGs的重要来源。采用O_(3)预氧化结合粉末活性炭(powdered activated carbon,PAC)与超滤(ultrafiltration,UF)组合工艺处理污水厂二级出水,考察O_(3)投加量与水样的pH、温度对二级出水中不同形态ARGs去除效果的影响因素和作用机理。结果表明:ARGs被O_(3)氧化失活后,经过PAC吸附与UF膜的过滤能得到更为有效的去除;在O_(3)最佳投加量(2.0 mg/L)条件下,O_(3)-PAC-UF组合工艺对二级出水中不同类型ARGs(tet A、tet C、tet G、sul I、sul II)和其他污染物(intⅠ1、16S rRNA)的去除量为10^(2.67)~10^(3.92)copies/mL,且对细胞态和游离态ARGs去除效果明显增强;升高pH和较低温度均利于ARGs的去除;O_(3)分子的直接氧化作用居于二级出水中ARGs去除机制的主导地位。综上,臭氧联合粉末活性炭与超滤组合可以有效去除二级出水中的抗生素抗性基因。ARGs(antibiotic resistance genes)as emerging environmental pollutants threaten human health,and studies show that secondary effluent from sewage treatment plants is an important source of ARGs in the environment.Ozone pre-oxidation combined with PAC(powdered activated carbon)and UF(ultrafiltration)was used to treat the secondary effluent from the wastewater treatment plant,and the factors and mechanisms of O_(3)dosage,pH,and temperature on the removal of different forms of ARGs in the secondary effluent were investigated.The results show that ARGs could be more effectively removed by PAC adsorption and UF after oxidative inactivation by O_(3);under the condition of optimal O_(3)dosage(2.0 mg/L),the combined O_(3)-PAC-UF process could remove different types of ARGs(tetA,tetC,tetG,sulI,sulII)and other pollutants(intⅠ1,16S rRNA)from the secondary effluent.The removal of different types of ARGs(tetA,tetC,tetG,sulI,sulII)and other pollutants(intⅠ1,16S rRNA)by the combined process is 10^(2.67)~10^(3.92) copies/mL,and the removal of cellular and free ARGs is significantly enhanced;the elevated pH and lower temperature facilitate the removal of ARGs;the direct oxidation of O_(3)molecules dominate the removal of ARGs in the secondary effluent.In conclusion,ozone combined with PAC and UF can effectively remove antibiotic resistance genes from secondary effluent.

关 键 词：抗生素抗性基因 二级出水 超滤 臭氧 粉末活性炭 

分 类 号：X703[环境科学与工程—环境工程]