西黄丸对犬乳腺肿瘤细胞及荷瘤裸鼠抗肿瘤效果研究  

Study on the Anti-tumour Effect of Xihuang Pill on Canine Mammary Tumour Cells and Tumor of Nude Mice

作　　者：要斌 黄靖 岳红 刘远清 李格宾[1] 王帅玉[1] YAO Bin;HUANG Jing;YUE Hong;LIU Yuan-qing;LI Ge-bin;WANG Shuai-yu(College of Veterinary Medicine,China Agricultural University,Beijing 100193,China)

机构地区：[1]中国农业大学动物医学院,北京100193

出　　处：《中国兽药杂志》2025年第1期21-28,共8页Chinese Journal of Veterinary Drug

摘　　要：为了探究西黄丸(XHP)在体内、体外抗乳腺肿瘤的作用,本实验采用CCK-8法测定XHP对犬乳腺肿瘤细胞系(CMT-U27)的细胞增殖抑制率;将荷瘤小鼠随机分为对照组(Control)、多柔比星组(DOX)、西黄丸低剂量组(XHP-L)、西黄丸中剂量组(XHP-M)和西黄丸高剂量组(XHP-H),考察不同剂量XHP对CMT-U27皮下移植瘤模型的抑瘤药效。体外试验结果显示,与对照组相比,XHP可显著抑制CMT-U27细胞的生长,并呈时间与浓度依赖性。动物试验结果显示,各试验组肿瘤体积显著小于对照组(P<0.05),西黄丸组肿瘤重量显著下降。其中,西黄丸低、中、高剂量组的抑瘤率分别为15.3%、20.53%和57.99%。与DOX组裸鼠相比,西黄丸组裸鼠精神良好,体重增长及脏器系数正常。综上所述,西黄丸对犬乳腺肿瘤细胞系CMT-U27有显著增殖抑制作用,对裸鼠皮下移植瘤具有良好的抑瘤效果,且对机体无明显毒副作用,为治疗犬乳腺肿瘤提供新的选择。To investigate the anti-mammary tumor effects of Xihuang pill(XHP)in vivo and in vitro,the CCK-8 assay was used to determine the inhibition rate of XHP on the cell proliferation of canine mammary tumor cell line(CMT-U27);the tumor-bearing mice were randomly divided into Control,Doxorubicin,Xihuang pill Low Dose(XHP-L),Xihuang pill Medium Dose(XHP-M),and XHP-H group to investigate the tumor inhibitory efficacy of different doses of XHP on the CMT-U27 subcutaneous transplantation tumor model.)and Xihuang pill high-dose group(XHP-H)to investigate the tumor-suppressive efficacy of different doses of XHP on the CMT-U27 subcutaneous graft tumor model.The results of in vitro tests showed that XHP significantly inhibited the growth of CMT-U27 cells in a time-and concentration-dependent manner compared with the control group.The results of animal tests showed that the tumor volume of each test group was significantly smaller than that of the control group(P<0.05),and the tumor weight of the XHP group was significantly decreased.Among them,the tumor inhibition rates of Xihuang pill low,medium and high dose groups were 15.3%,20.53%and 57.99%,respectively.Compared with the nude mice in the DOX group,the nude mice in the Xihuang pill group were in good spirits,with normal weight gain and organ coefficients.In conclusion,the Xihuang pill has a significant proliferation inhibitory effect on canine mammary tumor cell line CMT-U27,has a good tumor suppression effect on subcutaneous transplantation tumor in nude mice,and has no apparent toxic side effects on the organism,providing a new choice for the treatment of canine mammary tumors.

关 键 词：西黄丸 乳腺肿瘤 抗肿瘤 

分 类 号：S859.79[农业科学—临床兽医学]