Single-cell RNA sequencing reveals the diversity of the immunological landscape response to genital herpes  

作　　者：Siji Chen Jiang Zhu Chunting Hua Chenxi Feng Xia Wu Can Zhou Xianzhen Chen Boya Zhang Yaohan Xu Zeyu Ma Jianping He Na Jin Yinjing Song Stijn van der Veen Hao Cheng 

机构地区：[1]Department of Dermatology and Venereology,Sir Run-Run Shaw Hospital,Zhejiang University School of Medicine,Hangzhou 310016,China [2]Department of Microbiology,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,Zhejiang University School of Medicine,Hangzhou 310058,China

出　　处：《Virologica Sinica》2024年第6期860-874,共15页中国病毒学(英文版)

基　　金：supported by grants from the National Natural Science Foundation of China[grant number 82471846,82103740 and 82103743];Medical Science and Technology Project of Zhejiang Province[grant number 2022RC198].

摘　　要：Genital herpes(GH)is a common sexually transmitted disease,which is primarily caused by herpes simplex virus type 2(HSV-2),and continues to be a global health concern.Although our understanding of the alterations in immune cell populations and immunomodulation in GH patients is still limited,it is evident that systemic intrinsic immunity,innate immunity,and adaptive immunity play crucial roles during HSV-2 infection and GH reactivation.To investigate the mechanisms underlying HSV-2 infection and recurrence,single-cell RNA sequencing(scRNA-seq)was performed on immune cells isolated from the peripheral blood of both healthy individuals and patients with recurrent GH.Furthermore,the systemic immune response in patients with recurrent GH showed activation of classical monocytes,CD4þT cells,natural killer cells(NK cells),and plasmacytoid dendritic cells(pDCs),especially of genes associated with the Toll-like receptor signaling pathway and T cell activation.Circulating immune cells in GH patients show higher expression of genes associated with inflammation and antiviral responses both in the scRNA-Seq data set and in independent quantitative real-time polymerase chain reaction(qRT-PCR)analysis and ELISA experiments.This study demonstrated that localized genital herpes,resulting from HSV reactivation,may influence the functionality of circulating immune cells,suggesting a potential avenue for future research into the role of systemic immunity during HSV infection and recurrence.

关 键 词：Single-cell RNA sequencing(scRNA-seq) Genital herpes(GH) Herpes simplex virus(HSV) Immune response 

分 类 号：R392[医药卫生—免疫学]