Ferritin nanoparticle-based Nipah virus glycoprotein vaccines elicit potent protective immune responses in mice and hamsters  

作　　者：Shaohong Chen Xinghai Zhang Yanfeng Yao Shengdong Wang Kangyin Li Baoyue Zhang Tianxi Ye Li Chen Yan Wu Entao Li Bichao Xu Pei Zhang Xia Chuai Yong Ran Rui Gong Huajun Zhang Sandra Chiu 

机构地区：[1]CAS Key Laboratory of Special Pathogens and Biosafety,Wuhan Institute of Virology,Center for Biosafety Mega-Science,Chinese Academy of Sciences,Wuhan 430062,China [2]University of Chinese Academy of Sciences,Beijing 100049,China [3]Center for Biosafety Mega-Science,Wuhan Institute of Virology,Chinese Academy of Sciences,Wuhan 430071,China [4]Institutional Center for Shared Technologies and Facilities of Wuhan Institute of Virology,CAS,Center for Instrumental Analysis and Metrology,Wuhan 430062,China [5]Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230027,China [6]Key Laboratory of Anhui Province for Emerging and Reemerging Infectious Diseases,Hefei 230027,China [7]Department of Laboratory Medicine,The First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230027,China

出　　处：《Virologica Sinica》2024年第6期909-916,共8页中国病毒学(英文版)

基　　金：supported by the Strategic Priority Research Program of the Chinese Academy of Sciences(Grant No.XDB0490000).

摘　　要：Nipah virus(NiV)is a zoonotic paramyxovirus in the genus Henipavirus that is prevalent in Southeast Asia.NiV leads to severe respiratory disease and encephalitis in humans and animals,with a mortality rate of up to 75%.Despite the grave threat to public health and global biosecurity,no medical countermeasures are available for humans.Here,based on self-assembled ferritin nanoparticles(FeNPs),we successfully constructed two candidate FeNP vaccines by loading mammalian cells expressing NiV sG(residues 71–602,FeNP-sG)and Ghead(residues 182–602,FeNP-Ghead)onto E.coli-expressed FeNPs(FeNP-sG and FeNP-Ghead,respectively)through Spycatcher/Spytag technology.Compared with sG and Ghead alone,FeNP-sG and FeNP-Ghead elicited significant NiV specific neutralizing antibody levels and T-cell responses in mice,whereas the immune response in the FeNP-sG immunized group was greater than that in the FeNP-Ghead group.These results further demonstrate that sG possesses greater antigenicity than Ghead and that FeNPs can dramatically enhance immunogenicity.Furthermore,FeNP-sG provided 100%protection against NiV challenge in a hamster model when it was administered twice at a dose of 5μg/per animal.Our study provides not only a promising candidate vaccine against NiV,but also a theoretical foundation for the design of a NiV immunogen for the development of novel strategies against NiV infection.

关 键 词：Nipah virus(NiV) Ferritin nanoparticles Vaccine candidate 

分 类 号：R373[医药卫生—病原生物学]