银杏C2H2型锌指蛋白家族的全基因组鉴定及生物信息学分析  

Genome-wide Identification of C2H2-Type Zinc Finger Proteins of Ginkgo biloba and Bioinformatics Analysis

作　　者：李建阳 熊思微 李贺勤[1] 崔鹏 贺炳 LI Jianyang;XIONG Siwei;LI Heqin;CUI Peng;HE Bing(Qingdao Agricultural University,Qingdao,266109;Guangdong Laboratory for Lingnan Modern Agriculture,Genome Analysis Laboratory of the Ministry of Agriculture and Rural Area,Agricultural Genomics Institute at Shenzhen,Chinese Academy of Agricultural Sciences,Shenzhen,518120)

机构地区：[1]青岛农业大学,青岛266109 [2]中国农业科学院深圳农业基因组研究所,农业农村部基因组分析实验室,岭南现代农业广东实验室,深圳518120

出　　处：《基因组学与应用生物学》2024年第11期1723-1734,共12页Genomics and Applied Biology

基　　金：深圳市科技创新委员会项目(KQTD20180411143628272)资助。

摘　　要：在多种高等植物中,C2H2型锌指蛋白(C2H2-type zinc finger proteins,C2H2-ZFPs)作为重要的转录因子家族,已被证实在生长发育和逆境胁迫响应等生物学过程中发挥着重要作用。本研究在银杏(Ginkgo biloba)中鉴定了61个C2H2-ZFPs家族成员,并分析了它们的理化性质、染色体定位、共线性关系、启动子顺式调控元件和组织表达模式。依据系统发育分析将这些C2H2-ZFPs家族成员分为5个亚家族,均匀的地分布在12条染色体上。亚细胞定位结果显示,银杏C2H2-ZFPs家族成员主要定位于细胞核中;启动子顺式元件分析表明,银杏C2H2-ZFPs家族含有许多光响应元件、植物激素反应元件;组织表达模式分析显示,61个C2H2-ZFPs家族成员在不同组织中表达,表明它们可能在不同组织、生长发育不同时期中发挥作用。该研究为银杏C2H2-ZFPs家族提供较为详细的概述,并为进一步探索该家族成员的作用奠定研究基础。In a variety of higher plants,C2H2-type zinc finger proteins(C2H2-ZFPs),as an important family of transcription factors,have been shown to play important roles in vital biological processes such as growth and development and response to adversity stress.In this study,61 members of the C2H2-ZFPs family were identified in Ginkgo biloba and analyzed for their physicochemical properties,chromosomal localization,covariance,promoter cis-regulatory elements and tissue expression patterns.Based on phylogenetic analysis,these C2H2-ZFPs family members were categorized into five subfamilies,which were evenly distributed on 12 chromosomes.The results of subcellular localization showed that the Ginkgo biloba C2H2-ZFPs family members were mainly located in the nucleus;the analysis of promoter cis-elements showed that the Ginkgo biloba C2H2-ZFPs family contained many light-responsive elements and phytohormoneresponsive elements;and the analysis of the tissue expression pattern showed that 61 C2H2-ZFPs family members were expressed in different tissues,suggesting that they may play roles in different tissues and at different times of growth and development.This study provides a detailed overview of the C2H2-ZFPs family in Ginkgo biloba and lays the foundation for further exploration of the roles of this family.

关 键 词：银杏 C2H2 基因家族 启动子元件 表达模式 

分 类 号：Q943.2[生物学—植物学] S792.95[农业科学—林木遗传育种]