Identification of resistance to sugar beet damping-off physiological responses of the major cultivated varieties of sugar beet  

甜菜主栽品种立枯病抗性鉴定及其生理响应的研究

作　　者：LIU Jiahui XIE Wanting SUN Xinke CHEN Zhao OU Yongfang SI Yuhan LIU Yonglong WANG Lihua WANG Yuguang 刘佳慧;谢婉婷;孙新可;陈昭;欧泳芳;司毓菡;刘永龙;王丽华;王宇光(黑龙江大学现代农业与生态环境学院,哈尔滨150080)

机构地区：[1]College of Modern Agriculture and Ecological Environment,Heilongjiang University,Harbin 150080,China

出　　处：《黑龙江大学工程学报(中英俄文)》2024年第3期104-112,共9页Journal of Engineering of Heilongjiang University

基　　金：黑龙江省高校基本科研业务经费专项资助项目(2023-KYYWF-1503);国家现代农业产业技术体系“质量安全与营养品质评价”项目(CARS-17)

摘　　要：In order to enrich the germplasm resources of sugar beet resistant to damping-off and explore their physiological mechanisms in response to the disease,the previously isolated and purified Rhizoctonia solani were employed to inoculate 112 main sugar beet varieties.The emergence rate,morbidity,mortality rate,peroxidase(POD)activity were investigated.The results showed that 112 main sugar beet varieties were divided into six levels according to the incidence rate:disease resistance(≤40%),high resistance(40%<to≤50%),medium resistance(50%<to≤60%),medium sensitivity(60%<to≤70%),high sensitivity(70%<to≤80%)and disease susceptibility(>80%).On the basis of this classification,5 disease resistant varieties,9 highly resistant varieties,9 moderately resistant varieties,20 moderately susceptible varieties,21 highly susceptible varieties,and 48 susceptible varieties were classified.Moreover,it was found that POD of disease-resistant varieties was significantly different from that of susceptible varieties.This approach provides a theoretical basis for the rational breeding of sugar beet varieties resistant to sugar beet damping-off,and have certain reference value for the development of the domestic sugar beet planting industry.为丰富我国甜菜立枯病抗病种质资源,探究其响应立枯病的生理机制,研究利用前期分离纯化得到的立枯丝核菌对112种甜菜主栽品种进行接种,统计上述品种的出苗率、发病率及死亡率,并测定过氧化物酶(Peroxidase, POD)活性。结果表明,112种甜菜主栽品种根据抗性水平分为抗病、高抗、中抗、中感、高感、感病6个级别。抗病品种发病率≤40%,40%<高抗品种发病率≤50%,50%<中抗品种发病率≤60%,60%<中感品种发病率≤70%,70%<高感品种发病率≤80%,感病品种发病率>80%。依此标准划分出抗病品种5个,高抗品种9个,中抗品种9个,中感品种20个,高感品种21个,感病品种48个。此外,研究发现抗病品种的POD活性与感病品种具有显著差异。研究结果为合理选育抗立枯病甜菜品种提供理论参考,对甜菜选种育种以及国内甜菜种植产业的发展具有一定参考价值。

关 键 词：Sugar beets sugar beet damping-off Rhizoctonia solani resistant varieties 

分 类 号：S566[农业科学—作物学]