响应面法优化水浴法提取雪莲果叶黄酮及抗氧化活性研究  

Response Surface Methodology Optimization of Flavonoids Extraction from Snow Lotus Leaves by Water Bath Method and Study on Antioxidant Activity

作　　者：鲍龙 李杰 胡明虎 贺雨菲 BAO Long;LI Jie;HU Minghu;HE Yufei(Lianyungang Environmental Monitoring Center,Lianyungang,Jiangsu 222000,China;School of Food Science,Sichuan Tourism University,Chengdu,Sichuan 610100,China)

机构地区：[1]江苏省连云港环境监测中心,江苏连云港222000 [2]四川旅游学院食品学院,四川成都610100

出　　处：《农产品加工》2025年第2期70-75,共6页Farm Products Processing

摘　　要：采用响应面法优化水浴法提取雪莲果叶中的总黄酮。以其总黄酮提取率表征黄酮类化合物的含量,通过响应面试验对提取工艺条件进行优化。以ABTS+自由基的清除率为指标,探究雪莲果叶中总黄酮的抗氧化能力。结果表明,雪莲果叶中总黄酮的最佳提取工艺条件为雪莲果叶用量1 g,料液比1∶25,水浴温度70℃,水浴时间80 min,雪莲果叶中的黄酮提取率为5.5321%。此工艺条件下的雪莲果叶总黄酮具有抗氧化能力,与质量浓度呈正相关。在质量浓度为25μg/mL时,ABTS^(+)自由基清除率达到69.74%,具有较强的抗氧化性。响应面法优化水浴法提取雪莲果叶总黄酮具有较好的抗氧化能力,可作为天然的绿色抗氧化剂。In this experiment,the response surface method was used to optimize the extraction of total flavonoids from yacon leaves using the water bath method.The content of flavonoids was characterized by the extraction rate of total flavonoids,and the extraction conditions were optimized by response surface experiment.The antioxidant capacity of total flavonoids in yacon leaves was investigated by ABTS+scavenging rate.The results indicated that the optimal extraction process conditions for total flavonoids in yacon leaves were as follows yacon leaves 1 g,solid-liquid ratio 1∶25,water bath temperature 70℃,water bath time 80 min,the extraction rate of flavonoids in yacon leaves was 5.5321%.The total flavonoids of yacon leaves under this process condition had antioxidant capacity and were positively correlated with concentration.When the mass concentration was 25μg/mL,the ABTS^(+)scavenging rate reached 69.74%,which had strong antioxidant activity.Optimization of water bath extraction of total flavonoids from yacon leaves by response surface methodology had good antioxidant capacity and could be used as a natural green antioxidant.

关 键 词：雪莲果叶 总黄酮 水浴法 抗氧化 

分 类 号：R284.2[医药卫生—中药学]