丹酚酸B对高糖诱导的HK-2细胞线粒体功能障碍及纤维化表型转换的影响  

Effects of Salvianolic Acid B on Mitochondrial Dysfunction and Fibrous Phenotype Transformation in HK-2 Cells Induced by High Glucose

作　　者：董政委 杨康 郭晓红 赵换 DONG Zhengwei;YANG Kang;GUO Xiaohong;ZHAO Huan(The First Affiliated Hospital of Henan University of Chinese Medicine,Heart Center/National Regional(Traditional Chinese Medicine)Cardiovascular Diagnosis and Treatment Center,Zhengzhou 450000,China;The First Affiliated Hospital of Henan University of Chinese Medicine,Kidney disease Diagnosis and Treatment Center,Zhengzhou 450000,China;The First Affiliated Hospital of Shandong First Medical University(Shandong Province Qianfoshan Hospital),Jinan 250014,China;Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China)

机构地区：[1]河南中医药大学第一附属医院心脏中心/国家区域(中医)心血管诊疗中心,郑州450000 [2]河南中医药大学第一附属医院肾脏病诊疗中心,郑州450000 [3]山东第一医科大学第一附属医院(山东省千佛山医院),济南250014 [4]天津中医药大学,天津301617

出　　处：《世界科学技术-中医药现代化》2024年第12期3145-3152,共8页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：河南省卫生健康委国家中医临床研究基地科研专项(2022JDZX109):基于肾小管上皮细胞外泌体调控的PPARγ信号通路探讨补阳还五汤干预肾间质纤维化的作用机制,负责人:杨康。

摘　　要：目的探究丹酚酸B(Salvianolic acid B,SalB)对高糖诱导的肾小管上皮细胞(Human renal proximal tubule cells,HK-2)线粒体功能及纤维化表型的影响。方法通过CCK8检测评估SalB干预HK-2的剂量,结合qRT-PCR和Western blot以及线粒体荧光探针探究SalB对高糖诱导的HK-2细胞线粒体功能障碍和细胞纤维化表型转换的影响。结果与对照组相比,高糖诱导的HK-2细胞线粒体数目显著下降(P<0.01),高剂量的SalB可明显回调线粒体数目;与对照组相比,高糖诱导的HK-2细胞CV-ATP5A蛋白表达水平受到显著抑制(P<0.01),经低剂量和高剂量SalB均可显著增加CV-ATP5A的表达(P<0.01);与对照组相比,高糖诱导的HK-2细胞FN1、Coll3、α-SMA转录水平明显增加(P<0.05),给予SalB干预可明显降低FN1、Coll3、MMP9、α-SMA的转录水平;与对照组相比,高糖诱导的HK-2细胞FN1、Coll3、α-SMA蛋白水平明显增加,给予SalB干预可明显降低FN1和Coll3蛋白表达水平。结论丹参主要活性成分SalB具有改善高糖诱导的HK-2细胞线粒体功能障碍的作用,并有效抑制纤维化表型的转换。Objective To investigate the effect of Salvianolic acid B(SalB)on mitochondrial function and fibrosis phenotype of human renal proximal tubule cells(HK-2)induced by high glucose.Methods The dose of SalB in HK-2was evaluated by CCK8 detection,and the effects of SalB on mitochondrial dysfunction and phenotypic transformation of cell fibrosis induced by high glucose were explored by qRT-PCR,Western blot and mitochondrial fluorescence probe.Results Compared with the control group,the number of mitochondria in HK-2 cells induced by high glucose decreased significantly(P<0.01),and high dose of SalB could significantly reduce the number of mitochondria.Compared with the control group,the expression level of CV-ATP5A protein in HK-2 cells induced by high glucose was significantly inhibited(P<0.01),and the expression of CV-ATP5A was significantly increased by low and high doses of SalB(P<0.01).Compared with the control group,the transcription levels of FN1,Coll3 andα-SMA in HK-2 cells induced by high glucose were significantly increased(P<0.05),and the intervention of SalB could significantly reduce the transcription levels of FN1,Coll3,MMP9 and α-SMA.Compared with the control group,the levels of FN1,Coll3 andα-SMA protein in HK-2 cells induced by high glucose were significantly increased,and the expression levels of FN1and Coll3 protein could be significantly decreased by the intervention of SalB.Conclusion SalB,the main active component of Salvia miltiorrhiza,can improve the mitochondrial dysfunction of HK-2 cells induced by high glucose and effectively inhibit the phenotypic transformation of fibrosis.

关 键 词：丹酚酸B HK-2细胞 线粒体功能障碍 纤维化表型转换 

分 类 号：R285.5[医药卫生—中药学]