菊花CmMYB15-like基因抗旱性功能鉴定  

Drought Resistance Function Identification of Chrysanthemum CmMYB15-like Gene

作　　者：史志坤 张怡雪 宋子燕 张开明[1] 李永华[1] 李菲 SHI Zhikun;ZHANG Yixue;SONG Ziyan;ZHANG Kaiming;LI Yonghua;LI Fei(College of Landscape Architecture and Art,Henan Agricultural University/Zhengzhou Key Laboratory for Development and Utilization of Characteristic Landscape Plant Resources,Zhengzhou 450002)

机构地区：[1]河南农业大学风景园林与艺术学院/郑州市特色景观植物资源开发与利用重点实验室,郑州450002

出　　处：《植物遗传资源学报》2025年第2期369-379,共11页Journal of Plant Genetic Resources

基　　金：中国博士后科学基金(2022M721037);国家自然科学基金青年项目(32302597);河南省自然科学基金青年项目(232300420187)。

摘　　要：菊花(Chrysanthemum morifolium)具有重要的观赏与经济价值,干旱会降低其产量和品质,造成巨大经济损失。挖掘菊花中的抗旱基因,培育抗旱品种是解决上述问题的重要手段。MYB转录因子参与植物生长发育和响应非生物胁迫。本研究在拟南芥中异源表达菊花CmMYB15-like基因,通过观察干旱胁迫后的表型变化,测定相对含水量、丙二醛含量、可溶性糖含量、超氧化物歧化酶活性、木质素含量等指标,探究该基因的抗旱性功能。结果显示,过表达CmMYB15-like拟南芥的存活率、相对含水量、超氧化物歧化酶活性、可溶性糖含量较野生型显著升高,丙二醛含量较野生型显著下降;幼苗主根长度及侧根数目较野生型显著升高。此外,AtPAL2、AtPAL4、At4CL1等木质素合成相关基因表达显著上调,木质素含量显著提高。这些结果表明,CmMYB15-like参与拟南芥干旱胁迫反应,为菊花抗旱基因资源筛选和品种培育提供一定理论依据。Chrysanthemum(Chrysanthemum morifolium)has important ornamental and economic value.Drought reduces its yield and quality,resulting in great economic losses.It is an important means to solve the above problems to dig out drought resistant genes in chrysanthemum and cultivate drought resistant varieties.MYB transcription factors are involved in plant growth and development and response to abiotic stress.This study involved the heterologous expression of the CmMYB15-like gene from chrysanthemum in Arabidopsis,followed by the observation of phenotypic changes under drought stress.The investigation of drought resistance function of this gene included measurement of relative water content(RWC),malondialdehyde(MDA)content,soluble sugars(SS)content,superoxide dismutase(SOD)activity,and lignin content.The survival rate,relative water content,superoxide dismutase activity and soluble sugar content of overexpressed CmMYB15-like Arabidopsis thaliana were significantly increased compared with the wild type,while the malondialdehyde content was significantly decreased compared with the wild type.The length of taproot and number of lateral roots were significantly higher than those of wild type.In addition,AtPAL2,AtPAL4,At4CL1 and other lignin synthesisrelated genes were significantly up-regulated,and the lignin content was significantly increased.These results indicate that CmMYB15-like is involved in the drought stress response of Arabidopsis thaliana,which provides a theoretical basis for the selection of drought-resistant gene resources and breeding of chrysanthemum cultivars.

关 键 词：菊花 MYB转录因子 CmMYB15-like 拟南芥 抗旱 

分 类 号：S682.11[农业科学—观赏园艺]