人工虎骨粉改善脊柱骨关节炎大鼠脊柱小关节的退变及关节软骨免疫微环境  

Artificial tiger bone meal improves degeneration of spinal facet joints and immune microenvironment of articular cartilage in rats with spine osteoarthritis

作　　者：李雯 赵婧宜 胡雯 熊彬 唐超 Li Wen;Zhao Jingyi;Hu Wen;Xiong Bin;Tang Chao(Southwest Medical University,Luzhou 646000,Sichuan Province,China;Department of Orthopedics,Affiliated Hospital of Southwest Medical University,Luzhou 646000,Sichuan Province,China)

机构地区：[1]西南医科大学,四川省泸州市646000 [2]西南医科大学附属医院骨科,四川省泸州市646000

出　　处：《中国组织工程研究》2025年第26期5511-5519,共9页Chinese Journal of Tissue Engineering Research

摘　　要：背景:脊柱骨关节炎是以脊柱小关节、关节韧带和肌腱为主要表现的慢性炎症性疾病,是慢性腰痛的主要原因。人工虎骨粉具有抗炎和缓解疼痛的生物作用,因此有望为脊柱骨关节炎治疗提供新的策略。目的:探讨人工虎骨粉调控白细胞介素23/白细胞介素17(IL-23/IL-17)免疫轴炎症因子表达水平改善大鼠脊柱骨关节炎的分子作用机制。方法:60只大鼠随机分为空白对照组、模型组及人工虎骨粉低、中、高剂量组,每组12只。除空白对照组外,其余各组通过对大鼠腰椎关节囊关节腔内注射碘乙酸0.1 mL建立骨关节炎模型。造模15 d后人工虎骨粉低、中、高剂量组分别按120,240,480 mg/(kg·d)灌胃给药治疗,空白对照组、模型组给予等体积生理盐水灌胃。给药21d后,采用WesternBlot法测量大鼠脊柱关节软骨中白细胞介素17、白细胞介素23、基质金属蛋白酶3、趋化因子受体6的表达水平;ELISA法检测大鼠血清中相关炎症因子的表达水平;应用苏木精-伊红染色和番红O-固绿染色法观察大鼠关节损伤退变程度。结果与结论:①Western Blot检测结果显示,人工虎骨粉高剂量组大鼠的关节软骨中白细胞介素17、白细胞介素23、基质金属蛋白酶3、趋化因子受体6的表达水平较模型组显著降低(P<0.05);②ELISA检测结果显示,与模型组相比,人工虎骨粉中、高剂量组大鼠的血清白细胞介素17、肿瘤坏死因子α、白细胞介素1β、基质金属蛋白酶3水平下调效果显著(P<0.05);③苏木精-伊红染色和番红O-固绿染色显示,人工虎骨粉各剂量组大鼠滑膜炎症细胞浸润及增生情况较模型组明显改善,其中人工虎骨粉中、高剂量组病理炎症国际骨关节炎研究学会评分及Mankin’s评分显著降低(P<0.05);④提示人工虎骨粉可明显改善碘乙酸化学刺激引起的大鼠脊柱小关节的炎症及退变,其中高剂量组效果更显著,其机制可能是通过�BACKGROUND:Spine osteoarthritis is a chronic inflammatory disease of the spine,articular ligaments,and tendons,and is the main cause of chronic low back pain.Artificial tiger bone powder has anti-inflammatory and pain-relieving biological effects,so it is expected to provide a new strategy for targeted therapy of spinal osteoarthritis.OBJECTIVE:To investigate the mechanism of artificial tiger bone meal in regulating the expression level of interleukin-23/interleukin-17 immune axis in improving spine osteoarthritis in rats.METHODS:Sixty SD rats were randomly divided into blank control group,model group,artificial tiger bone meal low-,medium-and high-dose groups,with 12 rats in each group.Except for the blank control group,osteoarthritis models were established in the other groups by injecting 0.1 mL of iodoacetic acid into the joint cavity of the lumbar vertebral joint capsule of the rats.15 days after modeling,the artificial tiger bone meal low,medium and high dose groups were treated by intragastric administration of 120,240 and 480 mg/kg per day respectively.The blank control group and model group were given the same volume of normal saline by gavage.After 21 days of treatment,the expression levels of interleukin-17,interleukin-23,matrix metalloproteinase-3,and chemokine receptor-6 in rat spinal articular cartilage were measured by western blot assay.The expression levels of related inflammatory factors in rat serum were detected by ELISA.The degree of joint injury and degeneration in rats was observed by hematoxylin-eosin staining and safranin O-fast green staining.RESULTS AND CONCLUSION:(1)The results of western blot assay showed that the expression levels of interleukin 17,interleukin 23,matrix metalloproteinase 3,and chemokine receptor 6 in the articular cartilage of rats in the artificial tiger bone meal high-dose group were significantly lower than those in the model group(P<0.05).(2)ELISA results showed that compared with the model group,the artificial tiger bone meal medium-and high-dose groups had a si

关 键 词：脊柱骨关节炎 人工虎骨粉 IL-23/IL-17免疫轴 炎症 大鼠 工程化组织构建 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R684