机构地区:[1]长春中医药大学,吉林省长春市130117 [2]长春中医药大学附属医院,吉林省长春市130021
出 处:《中国组织工程研究》2025年第26期5549-5555,共7页Chinese Journal of Tissue Engineering Research
基 金:吉林省自然科学基金项目(YDZJ202201ZYTS214),项目负责人:丛德毓;国家自然科学基金项目(82274673),项目负责人:丛德毓。
摘 要:背景:研究发现,推拿能降低血糖、促进成肌因子的表达及提高骨骼肌含量。细胞外基质是骨骼肌的重要成分,推拿与细胞外基质的关联及作用机制尚不明确。目的:探究推拿对2型糖尿病肌少症大鼠细胞外基质胶原沉积的影响。方法:将24只Wistar雄性大鼠随机分为空白组、模型组和推拿组,采用高脂饲料结合链脲佐菌素方法建立2型糖尿病肌少症模型,造模成功后推拿组给予腹部结合后肢部推拿治疗。治疗8周后,检测大鼠空腹血糖、血清胰岛素水平,双能X射线检测骨骼肌质量,小动物跑步机检测力竭时间,斜板实验检测滑落角度,苏木精-伊红染色观察骨骼肌组织的病理改变,Masson染色观察骨骼肌胶原沉积情况,qPCR和Westernblot检测骨骼肌Ⅰ型胶原、Ⅲ型胶原mRNA和蛋白表达。结果与结论:①与模型组比较,推拿组大鼠血糖(P<0.05)、血清胰岛素(P<0.01)降低;②与模型组比较,推拿组大鼠骨骼肌质量、跑步力竭时间、斜板实验角度均增加(P<0.05);③与模型组比较,推拿组大鼠骨骼肌排列整齐,肌萎缩改善,胶原纤维沉积减少;④与模型组比较,推拿组大鼠骨骼肌Ⅰ型胶原、Ⅲ型胶原mRNA和蛋白表达降低(P<0.05);⑤结果表明,推拿可以提高胰岛素敏感性,降低2型糖尿病大鼠血糖水平,改善骨骼肌质量、力量和功能,减少胶原沉积,可能是推拿发挥治疗作用的潜在靶点。BACKGROUND:Studies have found that massage can reduce blood sugar,promote myogenic factor expression,and increase skeletal muscle content.The extracellular matrix is an important component of skeletal muscle,and association between massage and extracellular matrix and their mechanism of action are still unclear.OBJECTIVE:To explore the effect of massage on extracellular matrix collagen deposition in type 2 diabetic sarcopenia rats.METHODS:Totally 24 Wistar male rats were randomly divided into blank group,model group,and massage group.High-fat diet combined with the streptozotocin method was used to establish a type 2 diabetes mellitus and sarcopenia model.After successful model establishment,the massage group used abdominal massage combined with hind limbs.After 8 weeks of treatment,the fasting blood glucose and serum insulin levels of the rats were measured.The skeletal muscle mass was detected by dual-energy X-ray.The exhaustion time was measured by small animal treadmill.The sliding angle was measured by inclined board test.The pathological changes of skeletal muscle tissue were observed by hematoxylin-eosin staining.The skeletal muscle collagen deposition was observed by Masson staining.The mRNA and protein expressions of type Ⅰ and type Ⅲ collagen in skeletal muscle were detected by qPCR and western blot assay.RESULTS AND CONCLUSION:(1)Compared with the model group,the blood glucose(P<0.05)and serum insulin(P<0.01)decreased in the massage group.(2)Compared with the model group,the skeletal muscle mass,running exhaustion time,and the angle of inclined plate experiment were increased in massage group(P<0.05).(3)Compared with the model group,the skeletal muscles of the massage group were arranged neatly,muscle atrophy was improved,and collagen fiber deposition was reduced.(4)Compared with the model group,the expression levels of type I and typeⅢcollagen mRNA and protein in skeletal muscle were decreased in the massage group(P<0.05).(5)The results suggest that massage can enhance insulin sensitivity,lower
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