电针调控少弱精子症模型大鼠“下丘脑-垂体-睾丸轴”的作用机制  

Regulatory mechanism of electroacupuncture on hypothalamic-pituitary-testicular axis in oligospermic rats

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作  者:赵振宁 李开颖 杨楠 孙文静[1,2,3] 魏晓歌 穆静 马惠昇[1,2,3] Zhao Zhenning;Li Kaiying;Yang Nan;Sun Wenjing;Wei Xiaoge;Mu Jing;Ma Huisheng(College of Traditional Chinese Medicine,Ningxia Medical University,Yinchuan 750004,Ningxia Hui Autonomous Region,China;Ministry of Education Key Laboratory of Modernization of Traditional Medicine for Ethic Minorities in Ningxia,Ningxia Medical University,Yinchuan 750004,Ningxia Hui Autonomous Region,China;Ningxia Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of High-Incidence Diseases,Yinchuan 750004,Ningxia Hui Autonomous Region,China)

机构地区:[1]宁夏医科大学,中医学院,宁夏回族自治区银川市750004 [2]宁夏医科大学,宁夏少数民族医药现代化教育部重点实验室,宁夏回族自治区银川市750004 [3]宁夏区域高发病中西医结合防治研究重点实验室,宁夏回族自治区银川市750004

出  处:《中国组织工程研究》2025年第26期5563-5571,共9页Chinese Journal of Tissue Engineering Research

基  金:宁夏回族自治区重点研发项目(2021BEG02041),项目名称:针灸疗法调控“下丘脑-垂体-睾丸轴”治疗男性生殖功能障碍的腧穴配伍循证评价及临床研究,项目参与人:马惠昇。

摘  要:背景:电针能够通过刺激下丘脑、垂体、睾丸中的激素合成与释放治疗少弱精子症,但其作用机制复杂,并且电针疗效与穴位配伍思路关系密切。目的:探索电针疗法能否通过调节血清相关性激素水平改善下丘脑-垂体-睾丸轴功能状态,进而治疗腺嘌呤诱导的大鼠少弱精子症。方法:采用随机数字表法将50只成年雄性SD大鼠随机分为空白组、模型组、电针组、左卡尼汀组、非经非穴组,每组10只。除空白组外,其他4组大鼠给予200 mg/(kg·d)腺嘌呤灌胃,连续灌胃28 d,建立少弱精子症模型。造模结束后,电针组选取中极、关元、足三里、三阴交四穴给予电针治疗,非经非穴组选取非穴点3处进行电针治疗,左卡尼汀组给予左卡尼汀灌胃治疗,1次/d,持续治疗28 d。治疗结束后,检测各组大鼠肾脏和睾丸脏器系数,精子数量、存活率和活力率,肾脏与睾丸组织病理形态学变化,血清中睾酮、促性腺激素释放激素、卵泡刺激素、黄体生成素、雌二醇、抑制素B、泌乳素水平。结果与结论:①模型组大鼠肾脏脏器系数高于空白组、电针组、左卡尼汀组(P<0.05),非经非穴组大鼠肾脏脏器系数高于电针组(P<0.05),各组大鼠睾丸脏器系数比较差异无显著性意义(P>0.05);②与空白组比较,模型组大鼠精子数量、精子存活率与活力率均降低(P<0.05);与模型组比较,电针组、左卡尼汀组大鼠精子数量、精子存活率与活力率均升高(P<0.05);非经非穴组大鼠精子数量、精子存活率与活力率均低于电针组(P<0.05);③苏木精-伊红染色结果显示,模型组大鼠肾脏与睾丸组织损伤严重,电针组和左卡尼汀组大鼠肾脏与睾丸组织损伤程度轻于模型组,非经非穴组大鼠肾脏与睾丸组织损伤程度重于电针组;④与空白组比较,模型组大鼠血清中睾酮、促性腺激素释放激素、抑制素B水平均降低(P<0.05),卵泡刺激素、黄体生成素、雌BACKGROUND:Electroacupuncture can treat oligoasthenospermia by stimulating hormone synthesis and release in the hypothalamus,pituitary gland and testis,but its mechanism is complex,and the efficacy of electroacupuncture is closely related to the idea of acupoint compatibility.OBJECTIVE:To explore whether electroacupuncture therapy can improve the function status of hypothalamic-pituitary-testicular axis by adjusting the level of serum related sex hormones in rats,so as to treat adenine-induced oligoasthenospermia in rats.METHODS:Fifty adult male Sprague-Dawley rats were randomly divided into blank,model,electroacupuncture,L-carnitine,and non-meridian and non-acupuncture point groups,with 10 rats in each group.Except for the blank group,the rats in the other four groups were given 200 mg/(kg·d)adenine via intragastric administration for 28 days to establish oligoasthenospermia models.After modeling,the rats in the electroacupuncture group were given electroacupuncture treatment at Zhongji,Guanyuan,Zusanli and Sanyinjiao acupoints,the non-acupoint group was selected for electroacupuncture treatment at three non-acupoint points,and the rats in the L-carnitine group were given oral infusion of L-carnitine,once daily,for 28 continuous days.After treatment,the kidney and testicular organ coefficients were calculated.Sperm count,survival rate and motility rate were determined.Pathological morphological changes of kidney and testicular tissues were observed.Serum sex hormones testosterone,gonadotropin releasing hormone,follicle stimulating hormone,luteinizing hormone,estradiol,statin B and prolactin levels were detected in rats of each group.RESULTS AND CONCLUSION:(1)The renal organ coefficient in the model group was higher than that in the blank,electroacupuncture and L-carnitine groups(P<0.05),the renal organ coefficient in the non-acupoint group was higher than that in the electroacupuncture group(P<0.05),and there was no significant difference in the testicular organ coefficient among all groups(P>0.05).(2)Compared

关 键 词:电针 少弱精子症 下丘脑-垂体-睾丸轴 腺嘌呤 性激素 工程化组织构建 

分 类 号:R459.9[医药卫生—治疗学] R319[医药卫生—临床医学] R588.1

 

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