怀珍养肝胶囊对碘乙酸钠致膝骨关节炎模型小鼠的关节保护效应  

Protective effect of Huaizhen Yanggan Capsule on knee osteoarthritis induced by sodium iodoacetate in model mice

作　　者：孙海亮 庞坚[1,2,3] 史万忠[1] 石瑛[1,2,3] Sun Hailiang;Pang Jian;Shi Wanzhong;Shi Ying(Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Shi Traumatology Center,Shuguang Hospital Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China;Institute of Traumatology&Orthopedics,Shanghai Institute of Traditional Chinese Medicine,Shanghai 201203,China)

机构地区：[1]上海中医药大学附属曙光医院,上海市201203 [2]上海中医药大学附属曙光医院石氏伤科医学中心,上海市201203 [3]上海市中医药研究院骨伤科研究所,上海市201203

出　　处：《中国组织工程研究》2025年第26期5579-5587,共9页Chinese Journal of Tissue Engineering Research

基　　金：上海市“科技创新行动计划”生物医药科技支撑专项(22S21900700),项目负责人:史万忠;华东片区及市级中医专科筋骨病损专科联盟建设,项目负责人:石瑛。

摘　　要：背景:前期研究显示怀珍养肝胶囊有较好的抗炎、镇痛和抗氧化等作用,但具体药效产生机制和对膝骨关节炎的治疗效果尚未做详细阐述。目的:评价怀珍养肝胶囊的抗炎镇痛作用及其对膝骨关节炎模型小鼠的关节保护作用。方法:①实验一:以二甲苯致小鼠耳郭肿胀实验评价怀珍养肝胶囊对小鼠的抗炎作用,ELISA法检测耳郭组织中白细胞介素6和肿瘤坏死因子α水平,苏木精-伊红染色观察抗炎小鼠耳郭组织形态。②实验二:以小鼠热板镇痛实验评价怀珍养肝胶囊对小鼠的镇痛作用,ELISA法检测小鼠血清中5-羟色胺、前列腺素E2、缓激肽和β内啡肽水平。③实验三:以注射碘乙酸钠膝骨关节炎模型小鼠研究怀珍养肝胶囊对膝骨关节炎的治疗作用,采用步幅分析评价镇痛效果,总抗氧化能力试剂盒检测血清中总抗氧化能力,Micro-CT扫描分析骨微结构及骨体积分数,苏木精-伊红与番红O-固绿染色观察关节软骨组织形态,改良Mankin’s评分评估关节软骨损伤程度,免疫组织化学染色检测关节软骨中肿瘤坏死因子α、白细胞介素6和Ⅱ型胶原的表达。结果与结论:①实验一:与空白组比较,怀珍养肝胶囊能显著抑制小鼠耳郭肿胀度(P<0.05),降低耳郭组织肿瘤坏死因子α和白细胞介素6水平(P<0.05);②实验二:与空白组比较,怀珍养肝胶囊中、高剂量组能明显提高痛阈值(P<0.05),降低血清中5-羟色胺、前列腺素E2、缓激肽水平(P<0.05),升高β内啡肽水平(P<0.05);③实验三:与模型组相比,怀珍养肝胶囊组小鼠的步幅明显增大(P<0.05),血清总抗氧化能力显著升高(P<0.05),Micro-CT扫描显示软骨下骨破坏得到抑制,骨体积分数明显增加(P<0.05),苏木精-伊红染色与番红O-固绿染色观察到关节软骨表面较平整,改良Mankin’s评分明显下降(P<0.05),免疫组织化学染色观察到Ⅱ型胶原表达增加(P<0.05),白细胞介素6和肿瘤坏死�BACKGROUND:Previous studies have shown that Huaizhen Yanggan Capsule has anti-inflammatory,analgesic,and antioxidant effects,but the mechanism of drug efficacy and whether it has therapeutic effects on knee osteoarthritis have not been elaborated.OBJECTIVE:To evaluate the anti-inflammatory and analgesic effects of Huaizhen Yanggan Capsule and its protective effect on knee osteoarthritis model mice.METHODS:(1)Experiment 1:The anti-inflammatory effect of Huaizhen Yanggan Capsule on mice was evaluated by ear swelling induced by xylene.The levels of interleukin-6 and tumor necrosis factor-αin ear tissues were detected by ELISA.The morphology of ear tissues of anti-inflammatory mice was observed by hematoxylin-eosin staining.(2)Experiment 2:The analgesic effect of Huaizhen Yanggan Capsule on mice was evaluated by hot plate analgesia experiment.The levels of 5-hydroxytryptamine,prostaglandin E2,bradykinin,andβ-endorphin in serum of mice were detected by ELISA.(3)Experiment 3:The therapeutic effect of Huaizhen Yanggan Capsule on knee osteoarthritis was studied by injecting sodium iodoacetate into knee osteoarthritis model mice.The analgesic effect was evaluated by step analysis.The total antioxidant capacity kit was used to detect the total antioxidant capacity in serum.Micro-CT scan was used to analyze bone structure and bone volume fraction.The tissue morphology of articular cartilage was observed by hematoxylin-eosin staining and safranin O-fast green staining.The injury degree of articular cartilage was evaluated by modified Mankin’s score.The expression of tumor necrosis factorα,interleukin 6,and type II collagen in articular cartilage was detected by immunohistochemical staining.RESULTS AND CONCLUSION:(1)In experiment 1,compared with blank group,Huaizhen Yanggan Capsule group could significantly inhibit the degree of ear swelling(P<0.05),and decrease the levels of tumor necrosis factorαand interleukin 6 in ear tissue(P<0.05).(2)In experiment 2,compared with blank group,Huaizhen Yanggan Capsule medium and hig

关 键 词：怀珍养肝胶囊 河蚌多糖提取物 抗炎 镇痛 膝骨关节炎 软骨 软骨下骨 抗氧化 MICRO-CT 工程化组织构建 

分 类 号：R459.9[医药卫生—治疗学] R318[医药卫生—临床医学] R684.3