Mapping RANKL-and OPG-expressing cells in bone tissue:the bone surface cells as activators of osteoclastogenesis and promoters of the denosumab rebound effect  

作　　者：Bilal M.El-Masri Christina M.Andreasen Kaja S.Laursen Viktoria B.Kofod Xenia G.Dahl Malene H.Nielsen Jesper S.Thomsen Annemarie Bruel Mads S.Sorensen Lars J.Hansen Albert S.Kim Victoria E.Taylor Caitlyn Massarotti Michelle M.McDonald Xiaomeng You Julia F.Charles Jean-Marie Delaisse Thomas L.Andersen 

机构地区：[1]Department of Clinical Research,University of Southern Denmark,Odense,Denmark [2]Danish Spatial Imaging Consortium,University of Southern Denmark,Odense,Denmark [3]Department of Pathology,Odense University Hospital,Odense,Denmark [4]Department of Forensic Medicine,Aarhus University,Aarhus,Denmark [5]Department of Biomedicine,Aarhus University,Aarhus,Denmark [6]Department of Otorhinolaryngology–Head and Neck Surgery and Audiology,University Hospital of Copenhagen,Rigshospitalet,Copenhagen,Denmark [7]Skeletal Diseases Program,Garvan Institute of Medical Research,Sydney,NSW,Australia [8]Cancer Theme,School of Medical Sciences,Faculty of Medicine and Health,The University of Sydney,Sydney,Australia [9]Department of Orthopedic Surgery,Brigham and Women’s Hospital,Harvard Medical School,Boston,MA,USA

出　　处：《Bone Research》2024年第4期912-932,共21页骨研究(英文版)

基　　金：supported by Odense University Hospital’s Fund for Free Research (A5111);the Region of Southern Denmark’s Fund for Free Research (A955);the VELUX Foundations (VELUX25723);supported by the Novo Nordisk Foundation。

摘　　要：Denosumab is a monoclonal anti-RANKL antibody that inhibits bone resorption,increases bone mass,and reduces fracture risk.Denosumab discontinuation causes an extensive wave of rebound resorption,but the cellular mechanisms remain poorly characterized.We utilized in situ hybridization(ISH)as a direct approach to identify the cells that activate osteoclastogenesis through the RANKL/OPG pathway.ISH was performed across species,skeletal sites,and following recombinant OPG(OPG:Fc)and parathyroid hormone 1–34(PTH)treatment of mice.

关 键 词：RANKL MAPPING OPG 

分 类 号：R68[医药卫生—骨科学]