高盐对异养耦合硫自养工艺影响及四氢甲基嘧啶羧酸缓解策略  

IMPACT OF HIGH SALT ON HETEROTROPHIC COUPLED SULFUR AUTOTROPHIC(HSAD)PROCESS AND ECTOINE MITIGATION STRATEGY

作　　者：李冉[1] 侯雅男 李海波 刘志华[1] 韩懿[1] 张道虹 宋圆圆[1] 郭建博 黄聪 LI Ran;HOU Yanan;LI Haibo;LIU Zhihua;HAN Yi;ZHANG Daohong;SONG Yuanyuan;GUO Jianbo;HUANG Cong(Tianjin Key Laboratory of Aquatic Science and Technology,Tianjin Chengjian University,Tianjin 300384,China;Tianjin Institute of Industrial Biotechnology,Chinese Academy of Sciences,Tianjin 300308,China;Taizhou University,Taizhou 318000,China)

机构地区：[1]天津城建大学水质科学与技术重点实验室,天津300000 [2]中国科学院天津工业生物技术研究所,天津300308 [3]台州学院,浙江台州318000

出　　处：《环境工程》2024年第12期79-88,共10页Environmental Engineering

基　　金：低C/N高盐废水生物强化脱氮技术开发(KHX2023-129);天津市合成生物技术创新能力提升行动项目“有机废弃物生物制氢关键技术研发应用”(TSBICIP-CXRC-074);国家自然科学基金项目(52000134)。

摘　　要：日益增加的含盐废水排放给异养耦合硫自养(HSAD)工艺带来了挑战。混养反硝化微生物对高盐胁迫的响应机制和可行的缓解策略需要进一步探索。研究发现,2%盐度下反硝化脱氮效率可提高到99.42%,当盐度升高到6%时,HSAD的反硝化性能显著下降,NO^(-)_(3)-N去除率从95.77%下降到38.01%,硫自养反硝化工艺(SAD)的贡献率继续高于异养反硝化(HD)。高盐度导致还原性辅酶Ⅰ含量和三磷酸腺苷水平在6%盐度时分别下降了10.74%、46.6%和56.28%。此外,反硝化酶(硝酸还原酶和亚硝酸还原酶)活性的降低以及反硝化功能菌数量的减少也是导致高盐胁迫下HSAD反硝化受到抑制的重要因素。值得注意的是,在盐度为6%的条件下添加250 mg/L的四氢甲基嘧啶羧酸可缓解盐度胁迫,并通过促进胞外聚合物物质的分泌和提高HSAD微生物的代谢活性来提高HSAD的反硝化效率。微生物群落分析表明,与不添加四氢甲基嘧啶羧酸的情况相比,HD功能菌的丰度增加了3.99%,凸显了四氢甲基嘧啶羧酸对群落演替和稳定性的关键调节作用。该研究结果加深了对高盐废水对HSAD的抑制机制的认识,为硫基混养反硝化技术缓解盐胁迫提供了可行的技术方案。Increasing saline wastewater discharge poses a challenge to denitrification by heterotrophic and sulfur autotrophic denitrification(HSAD).The response mechanisms and feasible mitigation strategies of heterotrophic denitrifying microorganisms to high salt stress need to be further explored.In this study,it was found that a salinity of 2%increased denitrification efficiency.However,the denitrification performance of HSAD significantly decreased when salinity increased to 6%,NO_(3)^(-)-N removal decreased from 95.77%to 38.01%,and the contribution of sulfur autotrophic denitrification(SAD)continued to be higher than that of heterotrophic denitrification(HD).High salinity stimulation resulted in nicotinamide adenine dinucleotide content and adenosine triphosphate levels decreasing by 10.74%,46.6%and 56.28%respectively,at 6%salinity.In addition,the reduced activities of denitrifying enzymes(nitrate reductase and nitrite reductase)and the decrease in denitrifying functional bacteria were also important factors contributing to the inhibition of HSAD denitrification under high salt stress.Notably,adding 250 mg/L ectoine at 6%salinity alleviated the salinity stress,and enhanced the denitrification efficiency of HSAD by promoting the secretion of extracellular polymeric substances and increasing the metabolic activities of HSAD microorganisms.Microbial community analysis showed that the abundance of HD-functional bacteria increased by 3.99%compared to the case without ectoine,highlighting the key regulatory role of ectoine on community succession and stability.The results of this study deepened the understanding of the inhibition mechanism of HSAD by high-salt wastewater,and provided a feasible technical solution for sulfur-based mixotrophic denitrification technology to alleviate salt stress.

关 键 词：高盐废水 异养耦合硫自养(HSAD)工艺 脱氮 微生物代谢 四氢甲基嘧啶羧酸 微生物群落 

分 类 号：X70[环境科学与工程—环境工程]