PeNAC67-PeKAN2-PeSCL23 and B-class MADS-box transcription factors synergistically regulate the specialization process from petal to lip in Phalaenopsis equestris  

作　　者：Qingyu Xu Zhenyu Yang Yupeng Jia Rui Wang Qiyu Zhang Ruonan Gai Yiding Wu Qingyong Yang Guoren He Ju Hua Wu Feng Ming 

机构地区：[1]Development Centre of Plant Germplasm Resources,College of Life Sciences,Shanghai Normal University,Shanghai 200234,China [2]Shanghai Key Laboratory of Plant Molecular Sciences,College of Life Sciences,Shanghai Normal University,Shanghai 200234,China [3]National Key Laboratory of Crop Genetic Improvement,Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan,China [4]Hubei Key Laboratory of Agricultural Bioinformatics,College of Informatics,Huazhong Agricultural University,Wuhan,China

出　　处：《Molecular Horticulture》2024年第1期380-395,共16页分子园艺(英文)

基　　金：funded by National Key Research and Development Program(grant 2023YFD1000500;2018YFD000400);Shanghai Engineering Research Center of Plant Germplasm Resources(grant number 17DZ2252700).

摘　　要：The molecular basis of orchid flower development involves a specific regulatory program in which MADS-box transcription factors play a central role.The recent‘perianth code’model hypothesizes that two types of higher-order heterotetrameric complexes,namely SP complex and L complex,play pivotal roles in the orchid perianth organ formation.Therefore,we explored their roles and searched for other components of the regulatory network.Through the combined analysis for transposase-accessible chromatin with high-throughput sequencing and RNA sequencing of the lip-like petal and lip from Phalaenopsis equestris var.trilip,transcription factor-(TF)genes involved in lip development were revealed.PeNAC67 encoding a NAC-type TF and PeSCL23 encoding a GRAS-type TF were differentially expressed between the lip-like petal and the lip.PeNAC67 interacted with and stabilized PeMADS3,which positively regulated the development of lip-like petal to lip.PeSCL23 and PeNAC67 competitively bound with PeKAN2 and positively regulated the development of lip-like petal to petal by affecting the level of PeMADS3.PeKAN2 as an important TF that interacts with PeMADS3 and PeMADS9 can promote lip development.These results extend the‘perianth code’model and shed light on the complex regulation of orchid flower development.

关 键 词：PeNAC67 PeSCL23 PeKAN2 Lip development ‘Perianth code’model Phalaenopsis orchids 

分 类 号：O17[理学—数学]