RETN基因与骨密度及骨折的因果关系研究  

A Study on the Casual Relationship Between RETN Gene and BMD and Fracture

作　　者：罗海勇 庞伟伟 卢璇 杨俊骁 谭丽君[3] LUO Haiyong;PANG Weiwei;LU Xuan;YANG Junxiao;TAN Lijun(Hunan Institute of Traffic Engineering,Hengyang 421000,China;Xiangxing College,Hunan University of TCM,Xiangyin 410007,China;College of Life Science,Hunan Normal University,Changsha 410081,China;Department of Orthopedics,Xiangya Hospital,Central South University,Changsha 410008,China)

机构地区：[1]湖南交通工程学院,衡阳421000 [2]湖南中医药大学湘杏学院,湘阴410007 [3]湖南师范大学生命科学学院,长沙410081 [4]中南大学湘雅医院骨外科,长沙410008

出　　处：《激光生物学报》2024年第6期541-549,共9页Acta Laser Biology Sinica

摘　　要：骨质疏松症是一种最常见的全身性、代谢性的骨骼疾病。血清抵抗素(RETN)的浓度与骨密度(BMD)相关联,但是RETN与BMD和骨折是否存在因果关系仍不清楚。本研究采用孟德尔随机化(MR)分析RETN基因表达与BMD和骨折的因果关系,然后在股骨颈骨折(FNF)和骨关节炎(OA)样本的单细胞RNA测序数据中分析RETN基因的差异表达。MR分析发现,RETN高表达会导致BMD降低和骨折风险增加。单细胞测序分析表明,RETN在FNF组中表达上调,主要由中性粒细胞表达,其相关的炎症趋化因子CXCL8在FNF组中显著高表达。本研究提示,RETN在骨质疏松发病过程中发挥了重要作用。这一结论为骨质疏松的诊断和治疗提供了基础。Osteoporosis is the most common systemic and metabolic bone disease.The concentration of serum resistin(RETN)is associated with bone mineral density,but it is still unclear whether there is a causal relationship between RETN and bone density and fractures.This study used Mendelian randomization(MR)to analyze the causal relationship between RETN gene expression and bone mineral density and fractures,and then analyzed the differential expression of RETN gene in single-cell sequencing data of femeral neck fracture(FNF)and osteoarthritis(OA)samples.MR analysis showed that high expression of RETN was found to lead to decreased bone mineral density and increased risk of fractures.RETN expression was upregulated in the FNF group,and RETN was mainly expressed by neutrophils.The inflammatory chemokine CXCL8 related to RETN was significantly upregulated in the FNF group.This study suggested the role of RETN in the pathogenesis of osteoporosis,providing a basis for the diagnosis and treatment of osteoporosis.

关 键 词：骨质疏松症 抵抗素 孟德尔随机化分析 单细胞RNA测序 骨密度 

分 类 号：R714.257[医药卫生—妇产科学] Q341[医药卫生—临床医学]