差异microRNA表达与女性髋部脆性骨折患者骨量丢失的相关性研究  

Correlation between differential microRNA expression and bone loss in female patients with hip fragility fractures

作　　者：王啸[1] 曹录民 华俊[1] 徐又佳[1] 刘栋[1] WANG Xiao;CAO Lu-min;HUA Jun;XU You-jia;LIU Dong(Department of Orthopedics,The Second Affiliated Hospital of Soochow University,Suzhou,Jiangsu 215004,China)

机构地区：[1]苏州大学附属第二医院骨科,江苏苏州215004

出　　处：《中华骨质疏松和骨矿盐疾病杂志》2024年第6期574-579,共6页Chinese Journal Of Osteoporosis And Bone Mineral Research

基　　金：国家自然科学基金(82003473);苏州市科技计划项目(SYW2024091)。

摘　　要：目的通过临床统计和生物信息学方法探索绝经后女性髋部骨折患者骨量丢失的相关因素。方法微小RNA(microRNA,miRNA)和信使RNA(messenger RNA,mRNA)表达谱来源于基因公共数据库。对倍数改变最高的差异miRNA使用TargetScan和miRDB工具预测靶基因。共计纳入20例股骨粗隆间骨折女性患者,术中收集骨髓。验证miRNA和靶基因在骨髓中的差异表达。所有数据进行正态分布检验。对各指标行两变量间线性相关分析,将与骨密度相关的指标纳入多重线性回归方程,最终指标与骨密度行偏相关分析。结果公共数据库中高骨量和低骨量组间共筛选140个miRNA,其中miR-595的|log2-FC|值最高。最终预测337个miR-595靶基因,与GSE100609交集筛选出CLDND1。髋部骨折患者高骨量组中miR-595上调,CLDND1下调。两变量间线性相关分析显示,年龄、体重、铁蛋白、miR-595、CLDND1与骨密度显著相关。进一步的多重线性回归分析显示,年龄、miR-595与骨密度高度相关;控制年龄变量,对miR-595与骨密度行偏相关分析显示,两者显著相关。结论miR-595及其下游CLDND1可能与绝经女性骨量丢失相关。Objective To explore relative factors for bone loss in postmenopausal female hip fracture patients through clinical statistics and bioinformatics methods.Methods The microRNA(miRNA)and messenger RNA(mRNA)expression profiles were sourced from the Gene Expression Omnibus(GEO).Target genes for the differential miRNA with the highest fold change were predicted by using TargetScan and miRDB tools.A total of 20 female patients with intertrochanteric femural fractures were included,and bone marrow was collected during surgery.The differential expression of miRNA and target genes in bone marrow was verified.All data were tested for normal distribution.Linear correlation analysis was performed on each indicator between two variables,and the indicators related to bone density were included in the multiple linear regression equation,and the partial correlation analysis was performed between the final indicators and bone density.Results Totally 140 miRNAs were screened between high bone mass and low bone mass groups in the public database,with miR-595 having the highest|log2-FC|value.Finally,337 target genes were predicted,and CLDND1 was screened by intersecting with GSE100609.MiR-595 was upregulated and CLDND1 was downregulated in the high bone mass group of patients with hip fractures.Univariate correlation analysis showed a significant correlation between age,weight,ferritin,miR-595,CLDND1,and bone density.Further multiple linear regression analysis showed a high correlation between age,miR-595,and bone density.After controlling for age variables,partial correlation analysis between miR-595 and bone density showed that they were significantly correlated.Conclusion miR-595 and its downstream CLDND1 might be the independent relative factors for bone loss in postmenopausal women.

关 键 词：骨折 骨质疏松 微小RNA 生物信息学 

分 类 号：R681[医药卫生—骨科学]