陆地棉种质资源种子萌发期耐盐性综合评价  

Comprehensive Evaluation of Salt Tolerance of Upland Cotton Germplasm at Seeds Germination Stage

作　　者：罗静 杜珊珊 孙绘健 姚青青 何忠盛 王东力 LUO Jing;DU Shanshan;SUN Huijian;YAO Qingqing;HE Zhongsheng;WANG Dongli(Xinjiang Bayingol Mongolian Autonomous Prefecture Agricultural Science Research Institute,Korla,Xinjiang 841000)

机构地区：[1]新疆巴音郭楞蒙古自治州农业科学研究院,新疆库尔勒841000

出　　处：《中国农学通报》2025年第3期25-35,共11页Chinese Agricultural Science Bulletin

基　　金：新疆维吾尔自治区自然科学(地州)基金项目“棉花种子萌发期耐盐性综合评价及耐盐种质的筛选”(2021D01F28);新疆生产建设兵团第二师铁门关市科技计划项目“第二师机采棉滴水出苗关键技术研究与集成示范”(2023GG1501)。

摘　　要：为了解不同基因型陆地棉种质资源的耐盐程度,本研究利用0、50、100、150、200、300 mmol/L浓度的NaCl溶液对40份材料进行盐胁迫处理,测定种子萌发期的相关指标;并采用主成分分析和隶属函数分析对种子萌发期耐盐性进行综合评价。结果表明:(1)高浓度NaCl溶液胁迫下,发芽势、发芽率、下胚轴长、根长和单株鲜物质质量均呈下降趋势。(2)对在A4浓度下发芽率>50%的30份材料进行相关性分析、主成分分析和隶属函数综合评价,得到4份耐盐性强的材料,分别是‘邯8266’、‘日辉棉6号’、‘中R971708’和‘快育2号’。(3)通过聚类分析,在欧氏距离5处,可以将30份材料分为3类,第Ⅰ类有1份为耐盐材料,第Ⅱ类有18份中等耐盐材料;第Ⅲ类有11份敏盐材料。In order to understand the salt tolerance of different genotypes of upland cotton germplasm resources,this study subjected 40 samples to salt stress using NaCl solutions with concentrations of 0,50,100,150,200,and 300 mmol/L,and measured relevant indicators during the seed germination period.Principal component analysis and membership function analysis were used to comprehensively evaluate the salt tolerance of seeds during germination.The results showed that(1)under the stress of high concentration NaCl solution,germination potential,germination rate,hypocotyl length,root length and fresh matter mass per plant all showed a downward trend.(2)Correlation analysis,principal component analysis and membership function comprehensive evaluation were performed on 30 materials with germination rate>50%at A4 concentration,four materials with strong salt tolerance were obtained,namely‘Han 8266’,‘Rihui Mian 6’,‘Zhong R971708’and‘Kuaiyu 2’.(3)Through cluster analysis,at the Euclidean distance of 5,the 30 materials could be divided into 3 categories.There were 1 salt-tolerant material in Category I,18 moderately salt-tolerant materials in Category II,and 11 salt-sensitive materials in Category III.

关 键 词：陆地棉 种质资源 种子 NACL溶液 盐胁迫 主成分分析 隶属函数 聚类分析 

分 类 号：S326[农业科学—作物遗传育种]