水蜡A病毒丁香分离物的鉴定与全基因组序列分析  

Ligustrum Virus A Isolates from Syringa lindl:Identification and Whole Genome Sequence Analysis

作　　者：王昀轩 刘丽[1] 孙春辉 鲁书豪 梁宇卿 崔雯莉 高佳仪 李永强[1] WANG Yunxuan;LIU Li;SUN Chunhui;LU Shuhao;LIANG Yuqing;CUI Wenli;GAO Jiayi;LI Yongqiang(College of Bioscience and Resource Environment,Beijing University of Agriculture/Key Laboratory of Urban Agriculture(North China),Ministry of Agriculture and Rural Affairs,Beijing 102206)

机构地区：[1]北京农学院生物与资源环境学院/农业农村部华北都市农业重点实验室,北京102206

出　　处：《中国农学通报》2025年第3期107-113,共7页Chinese Agricultural Science Bulletin

基　　金：北京市教委科研项目“利用siRNA序列组装技术鉴定葡萄新病毒”(KM201610020008)。

摘　　要：研究旨在明确侵染北京丁香(Syringa lindl)并引起褪绿、花叶、卷叶症状的病毒种类,以及其基因组分子特征和进化关系。利用小RNA深度测序和RT-PCR技术对病样进行检测分析。深度测序结果显示病样中检测到水蜡A病毒(ligustrum virus A,LVA)并获得该病毒序列全长。北京丁香分离物LVANXY的基因组序列全长8488 nt,北京丁香分离物LVA-HLG的基因组序列全长8478 nt。序列一致性分析表明,LVA-NXY、LVA-HLG基因组序列与中国沈阳市的东北连翘分离物LVA-DBLQ基因组序列相似度最高。未在已报道的7个LVA分离物中检测到重组事件。基于基因组序列的系统发育树显示LVANXY、LVA-HLG与LVA-DBLQ、沈阳紫丁香分离物LVA-DX共聚同一分支中,显示出较近的亲缘关系。本研究报道了侵染北京丁香的水蜡A病毒基因组的全长序列,探究了病毒基因组分子特征及进化关系,为该病毒遗传变异研究及丁香病毒病的防控提供理论依据。To identify and characterize the candidate viruses infecting Beijing lilac(Syringa lindl)associated with the symptoms of leaf chlorosis,mosaic and leaf curling,small RNA deep sequencing and RT-PCR were conducted with the diseased leaf samples.Ligustrum virus A(LVA)was detected and the complete genome sequences of two isolates were obtained.The Beijing lilac isolate LVA-NXY is 8488 nt in length,while the isolate LVA-HLG is 8478 nt.Sequence similarity analysis showed that LVA-NXY and LVA-HLG were most similar to the LVA-DBLQ isolate from Forsythia suspensa in Shenyang,China.No recombination events were detected among the seven reported LVA isolates.The phylogenetic tree based on the genome sequences indicated that LVA-NXY and LVA-HLG clustered together with LVA-DBLQ and the Syringa vulgaris isolate LVA-DX in the same branch,showing a close phylogenetic relationship.This study reports the complete genome sequence of ligustrum virus A infecting Beijing lilac and explores the molecular characteristics and evolutionary relationships of the virus which provides a theoretical basis for the study of genetic variation and the control of lilac virus disease.

关 键 词：丁香 水蜡A病毒 小RNA 基因组 序列分析 分子特征 进化关系 

分 类 号：S432.1[农业科学—植物病理学]