干旱胁迫下甜菜种子萌发响应转录组分析  

Transcriptome Analysis of Sugar Beet Seed Germination Response Under Drought Stress

作　　者：岳开心 王佳琦[1] 刘乃新[1] YUE Kaixin;WANG Jiaqi;LIU Naixin(College of Advanced Agriculture and Ecological Environment,Heilongjiang University,Harbin 150080)

机构地区：[1]黑龙江大学现代农业与环境学院,哈尔滨150080

出　　处：《中国农学通报》2025年第3期114-122,共9页Chinese Agricultural Science Bulletin

基　　金：黑龙江省省属高等学校基本科研业务费项目(2020-KYYWF-1028);黑龙江省博士后“纳米硒生物强化调控寒地香稻产量和品质的分子机制研究”(LBH-Z22245);国家现代农业产业技术体系(糖料)建设项目“质量安全与营养品质评价”(CARS-170503)。

摘　　要：本研究旨在筛出甜菜中响应干旱胁迫的重要调控基因,为中国选育抗旱甜菜品种提供参考。以糖用甜菜‘KWS2314’种子为试材,对其萌发过程中的3个阶段进行转录组测序。通过WGCNA分析测得出关键基因模块。对差异表达基因进行GO分析后,发现植物应对干旱胁迫时,其差异基因的分子功能、细胞组分和生物学过程发生变化。在CK_(0)-vs-T_(1),CK_(0)-vs-T_(2),CK_(1)-vsT_(1)和CK_(1)-vss-T_(2)比较中发现分子功能的催化活性基因数量、细胞组成的细胞基因数量和生物学过程的代谢过程基因数量最多。在KEGG富集分析中,发现对照组CK_(0),CK_(1),CK_(2)和处理组T_(1),T_(2)之间的通路显著富集。频率最高的两种途径是代谢途径通路和次级代谢产物的生物合成通路,这表明了葡萄糖、脂肪、蛋白质的代谢和干旱有着密切联系。此项工作阐明了甜菜种子在缺水环境下萌发的分子机制,为植物抗旱研究奠定了理论依据。The aim of this study was to screen out important regulatory genes in sugar beet in response to drought stress,and to provide reference for the selection and breeding of drought-resistant sugar beet varieties in China.The transcriptome of sugar beet‘KWS2314’seeds was sequenced at three stages of the germination process.Key gene modules were measured by WGCNA analysis.GO analysis of differentially expressed genes revealed changes in the molecular functions,cellular components and biological processes of the differential genes in response to drought stress in plants.The highest number of catalytically active genes for molecular functions,cellular genes for cellular composition and genes for metabolic processes for biological processes were found in the comparison of CK_(0)-vs-T_(1),CK_(0)-vs-T_(2),CK_(1)-vsT_(1)and CK_(1)-vsT_(2).In KEGG enrichment analyses,significant enrichment was found for pathways between CK_(0),CK_(1),CK_(2)in the control group and T_(1),T_(2)in the treatment group.The two pathways with the highest frequencies were the metabolic pathway and the biosynthesis of secondary metabolites pathway,suggesting that the metabolism of glucose,fats,and proteins was closely linked to drought.This work elucidated the molecular mechanism of sugar beet seed germination under water deficit environment,and laid a theoretical basis for plant drought resistance research.

关 键 词：甜菜 干旱胁迫 萌发期 转录组 植物代谢 RNA提取 KEGG富集分析 GO富集分析 

分 类 号：S566.3[农业科学—作物学]