遮阴处理下睡莲‘甘娜’叶片转录组分析  

Transcriptome Analysis of Nymphaea‘Madame Ganna Walska’Leaves under Shading Treatment

作　　者：王亚坤 冯超越 滕云 岳建华 徐函兵 杨丽 Wang Yakun;Feng Chaoyue;Teng Yun;Yue Jianhua;Xu Hanbing;Yang Li(College of Horticulture,Xinyang Agriculture and Forestry University,Xinyang 464000,China)

机构地区：[1]信阳农林学院园艺学院,河南信阳464000

出　　处：《山东农业科学》2025年第1期12-21,共10页Shandong Agricultural Sciences

基　　金：河南省科技攻关计划项目(232102110184);河南省高等学校重点科研项目(23B180005)。

摘　　要：本研究对遮阴处理和自然光照下睡莲‘甘娜’叶片进行转录组分析,以揭示遮阴对其叶片发育的影响机制。结果表明,遮阴处理下共筛选得到1303个差异表达基因,其中558个基因上调,745个基因下调;GO功能注释分析发现,遮阴处理会在细胞发育和次生代谢等方面造成影响;KEGG通路注释与富集分析结果表明,遮阴处理会影响次生代谢、信号转导、细胞壁生物合成以及光合作用等信号通路的表达。差异表达基因筛选结果显示,遮阴条件下睡莲叶片生长素信号转导途径中的AUX1/LAX、SAUR、IAA基因,光合作用途径中的psbR、LHCB4、LHCB1基因,代谢途径中的CHS、4CL等基因,细胞壁发育中的GFPT、TUBA、CESA等基因以及光受体的基因如HY5、RFWD2、COP1等都存在显著差异表达。这表明遮阴处理下,睡莲叶片通过限制细胞壁生长,影响光吸收能力,调节初生以及次生代谢途径,调控对生长素的响应和光信号传导,以及调节相关基因的表达来适应环境变化,维持正常生长和生理功能。This study conducted transcriptome analysis on the leaves of Nymphaea‘Madame Ganna Walska’under shading treatment and natural light to reveal the influencing mechanism of shading on its leaf development.The results showed that a total of 1303 differentially expressed genes were screened under shading treatment,of which,558 genes were up-regulated and 745 genes were downregulated.GO functional annotation analysis found that shading treatment could affect cell development and secondary metabolism.The KEGG pathway annotation and enrichment analysis results showed that shading treatment affected the expression of signaling pathways such as secondary metabolism,signal transduction,cell wall biosynthesis and photosynthesis.Screening of differential expression gene showed that under shading conditions,the genes of AUX1/LAX,SAUR,and IAA in auxin signaling pathway,psbR,LHCB4 and LHCB1 in photosynthesis pathway,CHS and 4CL in metabolic pathways,GFPT,TUBA and CESA in cell wall development,as well as genes involved in photo morphogenesis such as HY5,RFWD2 and COP1,of Nymphaea leaves exhibited significantly differential expression.This indicated that under shading or low light conditions,Nymphaea leaves adapted to environmental change by limiting cell wall growth,affecting light absorption capacity,regulating primary and secondary metabolic pathways,responding to auxin,and regulating light signal transduction and related genes expression,so as to maintaining their normal growth and physiological functions.

关 键 词：遮阴 睡莲‘甘娜’ 转录组 代谢途径 

分 类 号：S682.32[农业科学—观赏园艺] Q781[农业科学—园艺学]