外源水杨酸对干旱胁迫下黄精种子萌发和幼苗抗氧化酶活性的影响  

Effect of Exogenous Salicylic Acid on Seed Germination and Antioxidant Enzyme Activity of Polygonatum sibiricum Seedlings under Drought Stress

作　　者：王志科 刘灵霞[1] Wang Zhike;Liu lingxia(School of Agriculture and Bioengineering,Longdong University/Gansu Key Laboratory of Protection and Utilization for Biological Resources and Ecological Restoration in Longdong,Qingyang 745000,China)

机构地区：[1]陇东学院农业与生物工程学院/甘肃省陇东生物资源保护利用与生态修复重点实验室,甘肃庆阳745000

出　　处：《山东农业科学》2025年第1期81-86,共6页Shandong Agricultural Sciences

基　　金：甘肃省自然科学基金项目(21JR11RM042);甘肃省高等学校创新基金项目(2021B-273);陇东学院博士基金计划项目(XYBY202007)。

摘　　要：为了探讨外源水杨酸(SA)对干旱胁迫下黄精种子萌发抑制的缓解作用,本试验以黄精种子为材料,研究0.01、0.10、1.00、2.00 mmol/L SA浸种对6%聚乙二醇(PEG-6000)胁迫(PG)下黄精种子萌发、幼苗生长和生理指标的影响。结果表明,6%PEG处理显著抑制黄精种子萌发和幼苗生长,发芽势、发芽指数、活力指数及幼苗芽长、根长、鲜重均显著下降。适宜浓度即0.10 mmol/L SA浸种处理显著提高PEG胁迫下黄精种子的发芽势、发芽指数和活力指数,显著促进幼苗芽和根的生长,幼苗芽和根的过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和过氧化物酶(POD)活性显著增强,丙二醛(MDA)含量显著降低,脯氨酸、可溶性糖含量显著增加。综上可知,0.10 mmol/L SA处理能显著减轻PEG胁迫对黄精种子萌发的抑制作用,显著提高黄精幼苗抗氧化酶活性,降低干旱胁迫造成的氧化损伤,有效提高黄精在种子萌发和幼苗生长阶段的抗旱性。To investigate the mitigation effect of exogenous salicylic acid(SA)on germination inhibition of seed under drought stress,seeds of Polygonatum sibiricum were used as material in the test,and the effects of soaking seeds with 0.01,0.10,1.00 and 2.00 mmol/L SA on seed germination,seedling growth and physiological indices under 6%polyethylene glycol(PEG-6000)stress(PG)were studied.The results showed that PG treatment significantly inhibited the seed germination and seedling growth of P.sibiricum showing as the decrease of germination potential,germination index,viability index,as well as seedling bud length,root length and fresh weight.Soaking seed with the suitable concentration of SA that was 0.10 mmol/L significantly improved the germination potential,germination index and vitality index of P.sibiricum seeds under PEG stress,significantly promoted the growth of seedling buds and roots,significantly enhanced the activities of catalase(CAT),superoxide dismutase(SOD)and peroxidase(POD)in buds and roots,significantly reduced the malondialdehyde(MDA)content,and significantly increased the contents of proline and soluble sugar.In conclusion,the treatment of 0.10 mmol/L exogenous SA could significantly reduce the inhibitory effect of PEG stress on germination of P.sibiricum seeds,significantly improve the antioxidant enzyme activities of P.sibiricum seedlings to reduce the oxidative damage caused by drought stress,and effectively improve the drought resistance of P.sibiricum at seed germination and seedling growth stages.

关 键 词：水杨酸 干旱胁迫 黄精 种子萌发 抗氧化酶 

分 类 号：S567.23[农业科学—中草药栽培]