番茄褐色皱纹果病毒检测及抗性材料筛选  

Detection of Tomato brown rugose fruit virus and Screening of Resistant Sources

作　　者：苏晓梅 吕宏君 刘淑梅[1] 王施慧[1] 高中强 侯丽霞[1] Su Xiaomei;Lyu Hongjun;Liu Shumei;Wang Shihui;Gao Zhongqiang;Hou Lixia(Institute of Vegetables,Shandong Academy of Agricultural Sciences/Key Laboratory of Huang-Huai Protected Horticulture Engineering,Ministry of Agriculture and Rural Affairs/Shandong Key Laboratory of Bulk Open-field Vegetable Breeding,Jinan 250100,China;Shandong Agricultural Technology Extension Center,Jinan 250100,China)

机构地区：[1]山东省农业科学院蔬菜研究所/农业农村部黄淮设施园艺工程重点实验室/山东省大宗露地蔬菜育种重点实验室,山东济南250100 [2]山东省农业技术推广中心,山东济南250100

出　　处：《山东农业科学》2025年第1期130-135,共6页Shandong Agricultural Sciences

基　　金：山东省农业良种工程项目(2023LZGC013,2022LZGC009);济南市农业应用技术创新计划项目(CX202101);中央引导地方科技发展专项资金项目(YDZX2022136)。

摘　　要：本研究对疑似番茄病毒病样本进行RT-PCR检测,并对扩增产物进行测序及系统进化分析,确认样本感染了番茄褐色皱纹果病毒(Tomato brown rugose fruit virus,ToBRFV)。染病番茄的种子中可检测到ToBRFV,而带毒种子长成的幼苗却检测不到ToBRFV,表明该病毒主要存在于种皮。利用10%的磷酸三钠对带毒种子进行消毒处理可以使病毒灭活。利用摩擦法对22份醋栗番茄进行苗期接种鉴定,获得了2份耐/抗ToBRFV的材料,可为抗性品种选育提供资源。In this study,the RT-PCR detection was carried out on suspected viral disease samples,and the amplified products were sequenced and the phylogenetic analysis was conducted,which verified that the samples were infected by Tomato brown rugose fruit virus(ToBRFV).The ToBRFV could be detected in seeds of infected tomato fruits,but could not be detected in seedings grown from the infected seeds,which indicated that ToBRFV was mainly existed in seed coat.The virus could be inactivated when the infected seeds were disinfected with 10%of trisodium phosphate.Twenty-two Solanum pimpinellifolium resources were inoculated and identified by mechanical inoculation method at seedling stage,and two materials tolerant or resistant to ToBRFV were obtained,which could provide resources for resistant variety breeding.

关 键 词：番茄褐色皱纹果病毒 分子检测 种子消毒 抗性鉴定 

分 类 号：S432.41[农业科学—植物病理学]