始兴石斛类原球茎诱导与植株再生  

Induction of Protocorm-like Bodies and Establishment of Plant Regeneration System in Dendrobium shixingense

作　　者：王玉 王可佳 邱绍聪 张应明[2] 段晓娟 王美娜 李健 陈建兵 陈朋 WANG Yu;WANG Kejia;QIU Shaocong;ZHANG Yingming;DUAN Xiaojuan;WANG Meina;LI Jian;CHEN Jianbing;CHEN Peng(The Orchid Conservation&Research Center of Shenzhen/Shenzhen Key Lab.for Orchid Conservation and Utilization/Key Lab.of National Forestry and Grassland Administration for Orchid Conservation and Utilization,Shenzhen 518114,China;Guangdong Chebaling National Nature Reserve Administration Bureau,Shaoguan 512500,China)

机构地区：[1]深圳市兰科植物保护研究中心/深圳市濒危兰科植物保护与利用重点实验室/兰科植物保护与利用国家林业与草原局重点实验室,广东深圳518114 [2]广东车八岭国家级自然保护区管理局,广东韶关512500

出　　处：《中国野生植物资源》2025年第1期60-64,79,共6页Chinese Wild Plant Resources

基　　金：深圳市科技计划项目——可持续发展科技专项(KCXFZ20211020164200001)。

摘　　要：目的:以始兴石斛茎段为外植体,建立始兴石斛的再生体系。方法:研究不同植物生长调节剂对始兴石斛愈伤、类原球茎和丛生芽的影响,并进行壮苗生根培养,获得大批量无菌组培苗。结果:1/2 MS基础培养基添加0.5 mg/L 6-BA和1.5 mg/L NAA可以诱导出纯粹的愈伤组织,添加2.0 mg/L 6-BA和0.5 mg/L NAA对愈伤、类原球茎和芽的混合发生诱导率较高;丛生芽最优增殖配方为添加2.0 mg/L KT和0.2 mg/L NAA的1/2 MS培养基,增殖系数可达10.05;选用降低了营养的1/4 MS培养基并添加0.2 mg/L NAA对生根壮苗效果最佳。结论:愈伤组织的诱导培养基最佳配方为1/2 MS+0.5 mg/L 6-BA+1.5 mg/L NAA,丛生芽最佳增殖配方为1/2 MS+2.0 mg/L KT+0.2 mg/L NAA,生根壮苗最佳配方为1/4 MS+0.2 mg/L NAA。Objective:To establish the regeneration system of Dendrobium shixingense using stem segments as materials.Methods:The effects of plant growth regulators on callus,protocorm-like bodies(PLBs)and cluster buds were studied.Rooting culture and seedling strengthening were carried out for a large number of seedlings.Results:Simple callus could be induced by 1/2 MS basal medium supplemented with 0.5 mg/L 6-BA and 1.5 mg/L NAA.The combination of callus,PLBs and adventitious buds had higher induction rate when supplemented with 2.0 mg/L 6-BA and 0.5 mg/L NAA.1/2 MS supplemented with 2.0 mg/L KT and 0.2 mg/L NAA was the most suitable medium for multiplication for shoots,and the multiplication coefficient could reach 10.05.1/4 MS supplemented with 0.2 mg/L NAA had the best effect on rooting and strengthening seedlings.Conclusion:The optimal medium for callus induction was 1/2 MS+0.5 mg/L 6-BA+1.5 mg/L NAA.The optimal medium for shoot proliferation was 1/2 MS+2.0 mg/L KT+0.2 mg/L NAA.The optimal medium for rooting and strengthening seedlings was 1/4 MS+0.2 mg/L NAA.

关 键 词：始兴石斛 愈伤 类原球茎 丛生芽 

分 类 号：R28[医药卫生—中药学]