推拿按揉法对坐骨神经损伤大鼠运动功能及NF-κB、c-Fos表达的影响  

Effects of Tuina Pressing and Kneading Method on Motor Function and Expression of NF-κB and c-Fos in Rats with Sciatic Nerve Injury

作　　者：张幻真 林志刚 陈水金 陈乐春 ZHANG Huanzhen;LIN Zhigang;CHEN Shuijin;CHEN Lechun(Rehabilitation Hospital,Fujian University of Traditional Chinese Medicine,Fuzhou,Fujian 350003,China)

机构地区：[1]福建中医药大学附属康复医院,福建福州350003

出　　处：《福建中医药》2024年第11期13-17,共5页Fujian Journal of Traditional Chinese Medicine

基　　金：福建省自然科学基金项目(2023J01880,2022J01881);福建省卫健委中青年人才骨干项目(2023GGA075);国家自然科学基金青年科学基金项目(82105039)。

摘　　要：目的观察推拿按揉法对坐骨神经损伤大鼠运动功能及核因子(NF)-κB、c-Fos表达的影响。方法采用随机数字表法将36只SPF级大鼠分为空白组、模型组和推拿组,每组12只。空白组未进行任何处理,模型组和推拿组建立坐骨神经慢性压迫性损伤大鼠模型。推拿组造模成功后第4天于近似“委中穴”处进行有节律地按压揉动,每次按揉10 min,每日1次,连续干预14 d。采用TreadScan步态分析系统检测造模前和干预7、14 d大鼠坐骨神经功能指数和步态参数;HE染色法观察大鼠坐骨神经病理改变;Western blot检测大鼠坐骨神经NF-κB、c-Fos蛋白表达量。结果HE染色结果显示:模型组大鼠坐骨神经纤维连续性差、排列紊乱,纤维间隙增大,部分髓鞘脱失,轴突肿胀、变性甚至断裂,炎性细胞分布增多;推拿组坐骨神经纤维排布较模型组整齐且紧密,可见部分髓鞘和轴突再生,炎性细胞减少。与空白组比较,模型组干预7、14 d坐骨神经功能指数以及患肢步幅、平均步行速度、站立相压力均明显降低(P<0.05),站立相时间明显缩短(P<0.05),坐骨神经NF-κB、c-Fos蛋白表达量均明显升高(P<0.05)。与模型组比较,推拿组干预7、14 d坐骨神经功能指数以及患肢步幅、平均步行速度和站立相压力均明显升高(P<0.05),站立相时间明显延长(P<0.05),坐骨神经NF-κB、c-Fos蛋白表达量均明显降低(P<0.05)。结论推拿可能通过下调NF-κB、c-Fos蛋白表达,减轻坐骨神经损伤后局部炎症水平,促进受损神经修复,从而改善患侧下肢运动功能。Objective:To observe the effect of tuina pressing and kneading method on motor function and the expression of NF-κB and c-Fos in rats with sciatic nerve injury.Methods:A total of thirty-six SPF rats were randomly divided into blank group,model group and tuina group,with 12 rats in each group.A rat model of chronic constriction injury was established in the model and tuina groups,while the blank group did not receive any intervention.On the fourth day after successful modeling,the tuina group rhythmically pressed and kneaded the area around the"Weizhong acupoint"for 10 minutes each time,once a day for 14 days,while the blank and model groups did not receive any intervention.The TreadScan gait analysis system was used to detect sciatic nerve function index and gait parameters in rats before modeling and at 7 and 14 days of intervention;HE staining method was used to observe pathological changes in the sciatic nerve;Western blot was used to detect the protein expression of NF-κB and c-Fos in the sciatic nerve.Results:The HE staining results showed that the sciatic nerve fibers in the model group had poor continuity,disordered arrangement,enlarged fiber gaps,partial demyelination,axonal swelling,degeneration,and even rupture,and increased distribution of inflammatory cells;the arrangement of sciatic nerve fibers in the tuina group was neat and tight compared to the model group,with some myelin sheaths and axons regenerating and a decrease in inflammatory cells.Compared with the blank group,the model group showed a significant decrease in sciatic nerve function index,affected limb stride,average walking speed,and standing pressure on the 7th and 14th day of intervention(P<0.05),a significant reduction in standing time(P<0.05),and a significant increase in sciatic nerve NF-κB and c-Fos protein expression(P<0.05).Compared with the model group,the tuina group showed a significant increase in sciatic nerve function index,affected limb stride,average walking speed,and standing pressure on the 7th and 14th day of interve

关 键 词：坐骨神经损伤 推拿 按揉 运动功能 NF-ΚB c-Fos 

分 类 号：R24[医药卫生—中医临床基础]